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. 1988 Jul 25;16(14B):7071–7085. doi: 10.1093/nar/16.14.7071

Eukaryotic DNA topoisomerase I reaction is topology dependent.

G Camilloni 1, E Di Martino 1, M Caserta 1, E di Mauro 1
PMCID: PMC338352  PMID: 2841648

Abstract

The effects of supercoiling on the topoisomerization reaction by eukaryotic DNA topoisomerases I have been analyzed. The systems used were: DNA topoisomerase I from wheat germ, chicken erythrocyte and calf thymus on a 2.3 kb DNA fragment which encompasses the immunoglobulin kappa-light chain (L kappa) promoter of the mouse plasmacytoma MPC11; S. cerevisiae DNA topoisomerase I on a 2.2 kb DNA fragment from the same organism which encompasses the regulatory and the coding region of the ADH II gene; wheat germ DNA topoisomerase I on the plasmid pUC18. It was found in every system that lack of torsional stress prevents topoisomerization of the substrate. A simple regulatory model of DNA topoisomerase I function, based on topological considerations, is presented.

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