Skip to main content
PMC home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1988 Oct 25;16(20):9477–9487. doi: 10.1093/nar/16.20.9477

A site-specific single strand endonuclease activity induced by NYs-1 virus infection of a Chlorella-like green alga.

Y N Xia 1, R Morgan 1, I Schildkraut 1, J L Van Etten 1
PMCID: PMC338757  PMID: 3186439

Abstract

A site-specific endonuclease was isolated from a eukaryotic Chlorella-like green alga infected with the dsDNA-containing virus NYs-1. The enzyme recognizes the sequence 5'-CC-3' and cleaves 5' to the first C. It cleaves 5'-CmC-3' sequences but not 5'-mCC-3' sequences. The enzyme creates breaks in dsDNA whenever two 5'-CC-3' sequences on opposite strands are close enough for the two strands to separate; when the 5'-CC-3' sequences on opposite strands are further apart only a portion of the strands separate. Consequently, NYs-1 endonuclease does not produce a completely stable DNA digestion pattern. The enzyme probably does not cleave ssDNA and definitely does not cleave ssRNA or dsRNA.

Full text

PDF
9481

Images in this article

9481Image
on p.9481
9483Image
on p.9483
9484Image
on p.9484

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blakesley R. W., Dodgson J. B., Nes I. F., Wells R. D. Duplex regions in "single-stranded" phiX174 DNA are cleaved by a restriction endonuclease from Haemophilus aegyptius. J Biol Chem. 1977 Oct 25;252(20):7300–7306. [PubMed] [Google Scholar]
  2. Burton W. G., Roberts R. J., Myers P. A., Sager R. A site-specific single-strand endonuclease from the eukaryote Chlamydomonas. Proc Natl Acad Sci U S A. 1977 Jul;74(7):2687–2691. doi: 10.1073/pnas.74.7.2687. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Jeppesen P. G. Separation and isolation of DNA fragments using linear polyacrylamide gradient gel electrophoresis. Methods Enzymol. 1980;65(1):305–319. doi: 10.1016/s0076-6879(80)65041-1. [DOI] [PubMed] [Google Scholar]
  4. Kuo T. T., Huang T. C., Teng M. H. 5-Methylcytosine replacing cytosine in the deoxyribonucleic acid of a bacteriophage for Xanthomonas oryzae. J Mol Biol. 1968 Jul 14;34(2):373–375. doi: 10.1016/0022-2836(68)90263-5. [DOI] [PubMed] [Google Scholar]
  5. Marinus M. G., Carraway M., Frey A. Z., Brown L., Arraj J. A. Insertion mutations in the dam gene of Escherichia coli K-12. Mol Gen Genet. 1983;192(1-2):288–289. doi: 10.1007/BF00327681. [DOI] [PubMed] [Google Scholar]
  6. McGraw R. A., 3rd Dideoxy DNA sequencing with end-labeled oligonucleotide primers. Anal Biochem. 1984 Dec;143(2):298–303. doi: 10.1016/0003-2697(84)90666-3. [DOI] [PubMed] [Google Scholar]
  7. Peacock A. C., Dingman C. W. Resolution of multiple ribonucleic acid species by polyacrylamide gel electrophoresis. Biochemistry. 1967 Jun;6(6):1818–1827. doi: 10.1021/bi00858a033. [DOI] [PubMed] [Google Scholar]
  8. Rogers S. G., Rhoades M. Bacteriophage T5-induced endonucleases that introduce site-specific single-chain interruptions in duplex DNA. Proc Natl Acad Sci U S A. 1976 May;73(5):1576–1580. doi: 10.1073/pnas.73.5.1576. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Schleif R. Assaying of organisms for the presence of restriction endonucleases. Methods Enzymol. 1980;65(1):19–23. doi: 10.1016/s0076-6879(80)65004-6. [DOI] [PubMed] [Google Scholar]
  11. Schuster A. M., Burbank D. E., Meister B., Skrdla M. P., Meints R. H., Hattman S., Swinton D., Van Etten J. L. Characterization of viruses infecting a eukaryotic Chlorella-like green alga. Virology. 1986 Apr 15;150(1):170–177. doi: 10.1016/0042-6822(86)90276-x. [DOI] [PubMed] [Google Scholar]
  12. Van Etten J. L., Burbank D. E., Schuster A. M., Meints R. H. Lytic viruses infecting a Chlorella-like alga. Virology. 1985 Jan 15;140(1):135–143. doi: 10.1016/0042-6822(85)90452-0. [DOI] [PubMed] [Google Scholar]
  13. Van Etten J. L., Schuster A. M., Girton L., Burbank D. E., Swinton D., Hattman S. DNA methylation of viruses infecting a eukaryotic Chlorella-like green alga. Nucleic Acids Res. 1985 May 24;13(10):3471–3478. doi: 10.1093/nar/13.10.3471. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Xia Y. N., Burbank D. E., Uher L., Rabussay D., Van Etten J. L. IL-3A virus infection of a Chlorella-like green alga induces a DNA restriction endonuclease with novel sequence specificity. Nucleic Acids Res. 1987 Aug 11;15(15):6075–6090. doi: 10.1093/nar/15.15.6075. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Xia Y. N., Burbank D. E., Uher L., Rabussay D., Van Etten J. L. Restriction endonuclease activity induced by PBCV-1 virus infection of a Chlorella-like green alga. Mol Cell Biol. 1986 May;6(5):1430–1439. doi: 10.1128/mcb.6.5.1430. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Xia Y. N., Narva K. E., Van Etten J. L. The cleavage site of the RsaI isoschizomer, CviII, is G decreases TAC. Nucleic Acids Res. 1987 Dec 10;15(23):10063–10063. doi: 10.1093/nar/15.23.10063. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Xia Y., Burbank D. E., Van Etten J. L. Restriction endonuclease activity induced by NC-1A virus infection of a Chlorella-like green alga. Nucleic Acids Res. 1986 Aug 11;14(15):6017–6030. doi: 10.1093/nar/14.15.6017. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES