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. 1988 Oct 25;16(20):9477–9487. doi: 10.1093/nar/16.20.9477

A site-specific single strand endonuclease activity induced by NYs-1 virus infection of a Chlorella-like green alga.

Y N Xia 1, R Morgan 1, I Schildkraut 1, J L Van Etten 1
PMCID: PMC338757  PMID: 3186439

Abstract

A site-specific endonuclease was isolated from a eukaryotic Chlorella-like green alga infected with the dsDNA-containing virus NYs-1. The enzyme recognizes the sequence 5'-CC-3' and cleaves 5' to the first C. It cleaves 5'-CmC-3' sequences but not 5'-mCC-3' sequences. The enzyme creates breaks in dsDNA whenever two 5'-CC-3' sequences on opposite strands are close enough for the two strands to separate; when the 5'-CC-3' sequences on opposite strands are further apart only a portion of the strands separate. Consequently, NYs-1 endonuclease does not produce a completely stable DNA digestion pattern. The enzyme probably does not cleave ssDNA and definitely does not cleave ssRNA or dsRNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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