Skip to main content
PMC home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1988 Oct 25;16(20):9557–9565. doi: 10.1093/nar/16.20.9557

Evidence for a gapped linear duplex DNA intermediate in the replicative cycle of human and simian spumaviruses.

J J Kupiec 1, J Tobaly-Tapiero 1, M Canivet 1, M Santillana-Hayat 1, R M Flügel 1, J Périès 1, R Emanoil-Ravier 1
PMCID: PMC338763  PMID: 2847117

Abstract

Two forms of linear DNAs have been found in simian (SFV1) and human (HSRV) spumaviruses: a linear duplex unsensitive to nuclease S1 and a sensitive structure with a single-stranded gap. Two nuclease S1 sensitive sites, mapping at the same position for both viruses, have been identified in the gapped structure. Using different molecular subgenomic clones of HSRV as probes in Southern blot analysis, one S1 site was localized in the 3'LTR and the other near the middle of the molecule at about 6.5 kbp from the 5' end of the viral genome. The latter site was shown to correspond to a single stranded region within the linear duplex DNA. Nucleotide sequence analysis revealed that the polypurine tract (PPT) usually found at the 5' boundary of the 3'LTR of retroviruses, is duplicated in HSRV at the 3' end of the pol gene, near the gap. This suggests that the synthesis of plus strand DNA is discontinuous, generating the gap.

Full text

PDF
9558

Images in this article

9560Image
on p.9560
9561Image
on p.9561

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Achong B. G., Mansell P. W., Epstein M. A., Clifford P. An unusual virus in cultures from a human nasopharyngeal carcinoma. J Natl Cancer Inst. 1971 Feb;46(2):299–307. [PubMed] [Google Scholar]
  2. Benzair A. B., Rhodes-Feuillette A., Emanoil-Ravicovitch R., Peries J. Characterization of RNase H activity associated with reverse transcriptase in simian foamy virus type 1. J Virol. 1983 Jul;47(1):249–252. doi: 10.1128/jvi.47.1.249-252.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Benzair A. B., Rhodes-Feuillette A., Emanoïl-Ravicovitch R., Peries J. Reverse transcriptase from simian foamy virus serotype 1: purification and characterization. J Virol. 1982 Nov;44(2):720–724. doi: 10.1128/jvi.44.2.720-724.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Benzair A. B., Rhodes-Feuillette A., Lasneret J., Emanoil-Ravier R., Peries J. Purification and characterization of simian foamy virus type I structural core polypeptides. Arch Virol. 1986;87(1-2):87–96. doi: 10.1007/BF01310545. [DOI] [PubMed] [Google Scholar]
  5. Benzair A. B., Rhodes-Feuillette A., Lasneret J., Emanoil-Ravier R., Périès J. Purification and characterization of the major envelope glycoprotein of simian foamy virus type 1. J Gen Virol. 1985 Jul;66(Pt 7):1449–1455. doi: 10.1099/0022-1317-66-7-1449. [DOI] [PubMed] [Google Scholar]
  6. Blum H. E., Harris J. D., Ventura P., Walker D., Staskus K., Retzel E., Haase A. T. Synthesis in cell culture of the gapped linear duplex DNA of the slow virus visna. Virology. 1985 Apr 30;142(2):270–277. doi: 10.1016/0042-6822(85)90335-6. [DOI] [PubMed] [Google Scholar]
  7. Cavalieri F., Rhodes-Feuillette A., Benzair A. B., Emanoïl-Ravicovitch R., Peries J. Biochemical characterization of simian foamy virus type i. Arch Virol. 1981;68(3-4):197–202. doi: 10.1007/BF01314572. [DOI] [PubMed] [Google Scholar]
  8. Evans T., Schon E., Gora-Maslak G., Patterson J., Efstratiadis A. S1-hypersensitive sites in eukaryotic promoter regions. Nucleic Acids Res. 1984 Nov 12;12(21):8043–8058. doi: 10.1093/nar/12.21.8043. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Feinberg A. P., Vogelstein B. "A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity". Addendum. Anal Biochem. 1984 Feb;137(1):266–267. doi: 10.1016/0003-2697(84)90381-6. [DOI] [PubMed] [Google Scholar]
  10. Flügel R. M., Rethwilm A., Maurer B., Darai G. Nucleotide sequence analysis of the env gene and its flanking regions of the human spumaretrovirus reveals two novel genes. EMBO J. 1987 Jul;6(7):2077–2084. doi: 10.1002/j.1460-2075.1987.tb02473.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Gianni A. M., Weinberg R. A. Partially single-stranded form of free Moloney viral DNA. Nature. 1975 Jun 19;255(5510):646–648. doi: 10.1038/255646a0. [DOI] [PubMed] [Google Scholar]
  12. Harris J. D., Scott J. V., Traynor B., Brahic M., Stowring L., Ventura P., Haase A. T., Peluso R. Visna virus DNA: discovery of a novel gapped structure. Virology. 1981 Sep;113(2):573–583. doi: 10.1016/0042-6822(81)90185-9. [DOI] [PubMed] [Google Scholar]
  13. Hess J. L., Pyper J. M., Clements J. E. Nucleotide sequence and transcriptional activity of the caprine arthritis-encephalitis virus long terminal repeat. J Virol. 1986 Nov;60(2):385–393. doi: 10.1128/jvi.60.2.385-393.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Hirt B. Selective extraction of polyoma DNA from infected mouse cell cultures. J Mol Biol. 1967 Jun 14;26(2):365–369. doi: 10.1016/0022-2836(67)90307-5. [DOI] [PubMed] [Google Scholar]
  15. Hooks J. J., Detrick-Hooks B. Simian foamy virus-induced immunosuppression in rabbits. J Gen Virol. 1979 Aug;44(2):383–390. doi: 10.1099/0022-1317-44-2-383. [DOI] [PubMed] [Google Scholar]
  16. Hooks J. J., Gibbs C. J., Jr The foamy viruses. Bacteriol Rev. 1975 Sep;39(3):169–185. doi: 10.1128/br.39.3.169-185.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Larsen A., Weintraub H. An altered DNA conformation detected by S1 nuclease occurs at specific regions in active chick globin chromatin. Cell. 1982 Jun;29(2):609–622. doi: 10.1016/0092-8674(82)90177-5. [DOI] [PubMed] [Google Scholar]
  18. Meinkoth J., Wahl G. Hybridization of nucleic acids immobilized on solid supports. Anal Biochem. 1984 May 1;138(2):267–284. doi: 10.1016/0003-2697(84)90808-x. [DOI] [PubMed] [Google Scholar]
  19. Neumann-Haefelin D., Schweizer M., Corsten B., Matz B. Detection and characterization of infectious DNA intermediates of a primary foamy virus. J Gen Virol. 1986 Sep;67(Pt 9):1993–1999. doi: 10.1099/0022-1317-67-9-1993. [DOI] [PubMed] [Google Scholar]
  20. Rethwilm A., Darai G., Rösen A., Maurer B., Flügel R. M. Molecular cloning of the genome of human spumaretrovirus. Gene. 1987;59(1):19–28. doi: 10.1016/0378-1119(87)90262-9. [DOI] [PubMed] [Google Scholar]
  21. Sonigo P., Alizon M., Staskus K., Klatzmann D., Cole S., Danos O., Retzel E., Tiollais P., Haase A., Wain-Hobson S. Nucleotide sequence of the visna lentivirus: relationship to the AIDS virus. Cell. 1985 Aug;42(1):369–382. doi: 10.1016/s0092-8674(85)80132-x. [DOI] [PubMed] [Google Scholar]
  22. Wain-Hobson S., Sonigo P., Danos O., Cole S., Alizon M. Nucleotide sequence of the AIDS virus, LAV. Cell. 1985 Jan;40(1):9–17. doi: 10.1016/0092-8674(85)90303-4. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES