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. 1988 Oct 25;16(20):9587–9596. doi: 10.1093/nar/16.20.9587

Characterization of DNA polymerase beta mRNA: cell-cycle and growth response in cultured human cells.

B Z Zmudzka 1, A Fornace 1, J Collins 1, S H Wilson 1
PMCID: PMC338765  PMID: 2460824

Abstract

DNA polymerase beta (beta-polymerase) is a housekeeping enzyme involved in DNA repair in vertebrate cells. We used a cDNA probe to study abundance of beta-polymerase mRNA in cultured human cells. The mRNA level in synchronized HeLa cells, representing different stages of the cell-cycle, varied only slightly. Contact inhibited fibroblasts AG-1522 contained the same level of mRNA as growing cells. The steady-state level of mRNA in fibroblasts is equivalent to 6 molecules per cell. The results indicate that the beta-polymerase transcript is "low abundance" and is neither cell-cycle nor growth phase responsive.

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Selected References

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