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. 1986 Feb 11;14(3):1293–1301. doi: 10.1093/nar/14.3.1293

Identification and characterisation of PmaCI an endonuclease of novel specificity from Pseudomonas maltophila.

J N Walker, P D Dean, J R Saunders
PMCID: PMC339504  PMID: 3005970

Abstract

We report the use of MonoQ FPLC (Fast Protein Liquid Chromatography) for the rapid purification of a novel Type II restriction endonuclease PmaCI, from Pseudomonas maltophila, which recognises the sequence 5'-CAC decreases GTG-3'. The resulting enzyme is free of other nucleases to a level suitable for its characterisation by multiple-substrate digestion and DNA sequencing techniques. This method appears to be widely applicable and we have used it for the isolation of restriction endonucleases of comparable purity from a range of other organisms. Also described is a rapid method for screening a library of small inserted regions in recombinant M13 molecules for the presence and subsequent screening of restriction sites of interest.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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