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. 1986 Apr 25;14(8):3153–3166. doi: 10.1093/nar/14.8.3153

Processing in the external transcribed spacer of ribosomal RNA from Physarum polycephalum.

B Blum, G Pierron, T Seebeck, R Braun
PMCID: PMC339739  PMID: 3010228

Abstract

The rDNA of the myxomycete Physarum polycephalum is transcribed to give a 13.3 kb precursor of ribosomal RNA. At 1.7 kb downstream of the primary initiation site there is a processing site or a second initiation site. This site was studied by S1-mapping, DNA sequencing and electron microscopy. None of these methods could conclusively distinguish between the two formal possibilities. However, capping experiments indicate that rapid processing is taking place at this site rather than reinitiation. In addition, primary transcripts and processed molecules were assayed throughout the synchronous mitotic cycle. During all interphase stages newly initiated transcripts of rDNA and products of the first processing step are present in similar amounts, indicating control of initiation and not of maturation as being the main regulatory step for the accumulation of mature rRNAs. During the brief period of mitosis the level of newly initiated rRNA precursors is lowered.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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