Skip to main content
PMC home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1986 Apr 25;14(8):3475–3485. doi: 10.1093/nar/14.8.3475

A 'hot-spot' for Ty transposition on the left arm of yeast chromosome III.

J R Warmington, R Anwar, C S Newlon, R B Waring, R W Davies, K J Indge, S G Oliver
PMCID: PMC339786  PMID: 3010239

Abstract

The small ring derivative of Saccharomyces cerevisiae chromosome III, which was formed by a cross-over between HML on the left arm and HMR on the right arm, contains three Ty elements. The class II element Ty 1-17 lies immediately centromere-distal to LEU2 on the left arm while two class I elements are tandemly arranged distal to PGK on the right arm. We have sequenced the regions of chromosome III surrounding Ty 1-17 and have defined a region where a number of transposition events have occurred. This region is flanked by the 5' ends of two tRNA genes, tRNA3Glu on the centromere distal side and tRNA3Leu immediately in front of LEU2. Close to the tRNA3Glu gene there is a region containing degenerate delta sequences organised in opposite orientations. Immediately distal to Ty 1-17 there are two complete solo delta elements, one inserted into the other. The sequence indicates that these two delta sequences were inserted into chromosome II by separate transposition events. A model is presented to explain how this structure arose and the role of solo delta elements in transposon propagation and maintenance is discussed.

Full text

PDF
3479

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andreadis A., Hsu Y. P., Kohlhaw G. B., Schimmel P. Nucleotide sequence of yeast LEU2 shows 5'-noncoding region has sequences cognate to leucine. Cell. 1982 Dec;31(2 Pt 1):319–325. doi: 10.1016/0092-8674(82)90125-8. [DOI] [PubMed] [Google Scholar]
  2. Cameron J. R., Loh E. Y., Davis R. W. Evidence for transposition of dispersed repetitive DNA families in yeast. Cell. 1979 Apr;16(4):739–751. doi: 10.1016/0092-8674(79)90090-4. [DOI] [PubMed] [Google Scholar]
  3. Cappello J., Cohen S. M., Lodish H. F. Dictyostelium transposable element DIRS-1 preferentially inserts into DIRS-1 sequences. Mol Cell Biol. 1984 Oct;4(10):2207–2213. doi: 10.1128/mcb.4.10.2207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chaleff D. T., Fink G. R. Genetic events associated with an insertion mutation in yeast. Cell. 1980 Aug;21(1):227–237. doi: 10.1016/0092-8674(80)90130-0. [DOI] [PubMed] [Google Scholar]
  5. Deininger P. L. Random subcloning of sonicated DNA: application to shotgun DNA sequence analysis. Anal Biochem. 1983 Feb 15;129(1):216–223. doi: 10.1016/0003-2697(83)90072-6. [DOI] [PubMed] [Google Scholar]
  6. Devenish R. J., Newlon C. S. Isolation and characterization of yeast ring chromosome III by a method applicable to other circular DNAs. Gene. 1982 Jun;18(3):277–288. doi: 10.1016/0378-1119(82)90166-4. [DOI] [PubMed] [Google Scholar]
  7. Eigel A., Feldmann H. Ty1 and delta elements occur adjacent to several tRNA genes in yeast. EMBO J. 1982;1(10):1245–1250. doi: 10.1002/j.1460-2075.1982.tb00020.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Farabaugh P. J., Fink G. R. Insertion of the eukaryotic transposable element Ty1 creates a 5-base pair duplication. Nature. 1980 Jul 24;286(5771):352–356. doi: 10.1038/286352a0. [DOI] [PubMed] [Google Scholar]
  9. Fulton A. M., Mellor J., Dobson M. J., Chester J., Warmington J. R., Indge K. J., Oliver S. G., de la Paz P., Wilson W., Kingsman A. J. Variants within the yeast Ty sequence family encode a class of structurally conserved proteins. Nucleic Acids Res. 1985 Jun 11;13(11):4097–4112. doi: 10.1093/nar/13.11.4097. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gafner J., Philippsen P. The yeast transposon Ty1 generates duplications of target DNA on insertion. Nature. 1980 Jul 24;286(5771):414–418. doi: 10.1038/286414a0. [DOI] [PubMed] [Google Scholar]
  11. Genbauffe F. S., Chisholm G. E., Cooper T. G. Tau, sigma, and delta. A family of repeated elements in yeast. J Biol Chem. 1984 Aug 25;259(16):10518–10525. [PubMed] [Google Scholar]
  12. Kingsman A. J., Gimlich R. L., Clarke L., Chinault A. C., Carbon J. Sequence variation in dispersed repetitive sequences in Saccharomyces cerevisiae. J Mol Biol. 1981 Feb 5;145(4):619–632. doi: 10.1016/0022-2836(81)90306-5. [DOI] [PubMed] [Google Scholar]
  13. Klar A. J., Strathern J. N., Hicks J. B., Prudente D. Efficient production of a ring derivative of chromosome III by the mating-type switching mechanism in Saccharomyces cerevisiae. Mol Cell Biol. 1983 May;3(5):803–810. doi: 10.1128/mcb.3.5.803. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Nasmyth K. A., Tatchell K., Hall B. D., Astell C., Smith M. A position effect in the control of transcription at yeast mating type loci. Nature. 1981 Jan 22;289(5795):244–250. doi: 10.1038/289244a0. [DOI] [PubMed] [Google Scholar]
  15. Olson M. V., Page G. S., Sentenac A., Piper P. W., Worthington M., Weiss R. B., Hall B. D. Only one of two closely related yeast suppressor tRNA genes contains an intervening sequence. Nature. 1981 Jun 11;291(5815):464–469. doi: 10.1038/291464a0. [DOI] [PubMed] [Google Scholar]
  16. Pearston D. H., Gordon M., Hardman N. Transposon-like properties of the major, long repetitive sequence family in the genome of Physarum polycephalum. EMBO J. 1985 Dec 16;4(13A):3557–3562. doi: 10.1002/j.1460-2075.1985.tb04117.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Sandmeyer S. B., Olson M. V. Insertion of a repetitive element at the same position in the 5'-flanking regions of two dissimilar yeast tRNA genes. Proc Natl Acad Sci U S A. 1982 Dec;79(24):7674–7678. doi: 10.1073/pnas.79.24.7674. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Staden R., McLachlan A. D. Codon preference and its use in identifying protein coding regions in long DNA sequences. Nucleic Acids Res. 1982 Jan 11;10(1):141–156. doi: 10.1093/nar/10.1.141. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Strathern J. N., Newlon C. S., Herskowitz I., Hicks J. B. Isolation of a circular derivative of yeast chromosome III: implications for the mechanism of mating type interconversion. Cell. 1979 Oct;18(2):309–319. doi: 10.1016/0092-8674(79)90050-3. [DOI] [PubMed] [Google Scholar]
  21. Warmington J. R., Waring R. B., Newlon C. S., Indge K. J., Oliver S. G. Nucleotide sequence characterization of Ty 1-17, a class II transposon from yeast. Nucleic Acids Res. 1985 Sep 25;13(18):6679–6693. doi: 10.1093/nar/13.18.6679. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. del Rey F. J., Donahue T. F., Fink G. R. sigma, a repetitive element found adjacent to tRNA genes of yeast. Proc Natl Acad Sci U S A. 1982 Jul;79(13):4138–4142. doi: 10.1073/pnas.79.13.4138. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES