Skip to main content
PMC home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1987 Feb 11;15(3):1097–1107. doi: 10.1093/nar/15.3.1097

A new type of plasmid from a wild isolate of Dictyostelium species: the existence of closely situated long inverted repeats.

H Orii, K Suzuki, Y Tanaka, K Yanagisawa
PMCID: PMC340510  PMID: 3029696

Abstract

A circular plasmid having high copy number was found in a wild isolate of Dictyostelium species. Gel electrophoresis, electron microscopy and Southern blot hybridization revealed that the plasmid, named pDG1, is 4.5 Kb (1.5 micron) in size with closely situated long inverted repeats. The plasmid seems to be located in the nuclei. It was not a derivative of ribosomal DNA. The possible correlation of the plasmid with the putative intermediate DNA of retrotransposon DIRS-1 found in Dictyostelium discoideum is discussed.

Full text

PDF
1100

Images in this article

1100Image
on p.1100
1101Image
on p.1101
1102Image
on p.1102
1103Image
on p.1103

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cappello J., Handelsman K., Lodish H. F. Sequence of Dictyostelium DIRS-1: an apparent retrotransposon with inverted terminal repeats and an internal circle junction sequence. Cell. 1985 Nov;43(1):105–115. doi: 10.1016/0092-8674(85)90016-9. [DOI] [PubMed] [Google Scholar]
  2. Collins J. Instability of palindromic DNA in Escherichia coli. Cold Spring Harb Symp Quant Biol. 1981;45(Pt 1):409–416. doi: 10.1101/sqb.1981.045.01.055. [DOI] [PubMed] [Google Scholar]
  3. Datta S., Gomer R. H., Firtel R. A. Spatial and temporal regulation of a foreign gene by a prestalk-specific promoter in transformed Dictyostelium discoideum. Mol Cell Biol. 1986 Mar;6(3):811–820. doi: 10.1128/mcb.6.3.811. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Firtel R. A., Cockburn A., Frankel G., Hershfield V. Structural organization of the genome of Dictyostelium discoideum: analysis by EcoR1 restriction endonuclease. J Mol Biol. 1976 Apr 25;102(4):831–852. doi: 10.1016/0022-2836(76)90294-1. [DOI] [PubMed] [Google Scholar]
  5. Firtel R. A., Lodish H. F. A small nuclear precursor of messenger RNA in the cellular slime mold Dictyostelium discoideum. J Mol Biol. 1973 Sep 15;79(2):295–314. doi: 10.1016/0022-2836(73)90007-7. [DOI] [PubMed] [Google Scholar]
  6. Firtel R. A., Silan C., Ward T. E., Howard P., Metz B. A., Nellen W., Jacobson A. Extrachromosomal replication of shuttle vectors in Dictyostelium discoideum. Mol Cell Biol. 1985 Nov;5(11):3241–3250. doi: 10.1128/mcb.5.11.3241. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Flavell A. J., Ish-Horowicz D. Extrachromosomal circular copies of the eukaryotic transposable element copia in cultured Drosophila cells. Nature. 1981 Aug 13;292(5824):591–595. doi: 10.1038/292591a0. [DOI] [PubMed] [Google Scholar]
  8. Goodchild J., Michniewicz J., Seto-Young D., Narang S. A novel deletion found during cloning of a synthetic palindromic DNA. Gene. 1985;33(3):367–371. doi: 10.1016/0378-1119(85)90246-x. [DOI] [PubMed] [Google Scholar]
  9. Junakovic N., Ballario P. Circular extrachromosomal copia-like transposable elements in Drosophila tissue culture cells. Plasmid. 1984 Mar;11(2):109–115. doi: 10.1016/0147-619x(84)90016-7. [DOI] [PubMed] [Google Scholar]
  10. Marko M. A., Chipperfield R., Birnboim H. C. A procedure for the large-scale isolation of highly purified plasmid DNA using alkaline extraction and binding to glass powder. Anal Biochem. 1982 Apr;121(2):382–387. doi: 10.1016/0003-2697(82)90497-3. [DOI] [PubMed] [Google Scholar]
  11. Metz B. A., Ward T. E., Welker D. L., Williams K. L. Identification of an endogenous plasmid in Dictyostelium discoideum. EMBO J. 1983;2(4):515–519. doi: 10.1002/j.1460-2075.1983.tb01456.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Michel F., Lang B. F. Mitochondrial class II introns encode proteins related to the reverse transcriptases of retroviruses. Nature. 1985 Aug 15;316(6029):641–643. doi: 10.1038/316641a0. [DOI] [PubMed] [Google Scholar]
  13. Nellen W., Firtel R. A. High-copy-number transformants and co-transformation in Dictyostelium. Gene. 1985;39(2-3):155–163. doi: 10.1016/0378-1119(85)90309-9. [DOI] [PubMed] [Google Scholar]
  14. Noegel A., Metz B. A., Williams K. L. Developmentally regulated transcription of Dictyostelium discoideum plasmid Ddp1. EMBO J. 1985 Dec 30;4(13B):3797–3803. doi: 10.1002/j.1460-2075.1985.tb04150.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Noegel A., Welker D. L., Metz B. A., Williams K. L. Presence of nuclear associated plasmids in the lower eukaryote Dictyostelium discoideum. J Mol Biol. 1985 Sep 20;185(2):447–450. doi: 10.1016/0022-2836(85)90416-4. [DOI] [PubMed] [Google Scholar]
  16. Reymond C. D., Nellen W., Firtel R. A. Regulated expression of ras gene constructs in Dictyostelium transformants. Proc Natl Acad Sci U S A. 1985 Oct;82(20):7005–7009. doi: 10.1073/pnas.82.20.7005. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  18. Ruan K., Emmons S. W. Extrachromosomal copies of transposon Tc1 in the nematode Caenorhabditis elegans. Proc Natl Acad Sci U S A. 1984 Jul;81(13):4018–4022. doi: 10.1073/pnas.81.13.4018. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Zuker C., Cappello J., Lodish H. F., George P., Chung S. Dictyostelium transposable element DIRS-1 has 350-base-pair inverted terminal repeats that contain a heat shock promoter. Proc Natl Acad Sci U S A. 1984 May;81(9):2660–2664. doi: 10.1073/pnas.81.9.2660. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES