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. 1987 Apr 10;15(7):2891–2909. doi: 10.1093/nar/15.7.2891

Immobilization of DNA via oligonucleotides containing an aldehyde or carboxylic acid group at the 5' terminus.

J N Kremsky, J L Wooters, J P Dougherty, R E Meyers, M Collins, E L Brown
PMCID: PMC340705  PMID: 3562241

Abstract

A general method for the immobilization of DNA through its 5'-end has been developed. A synthetic oligonucleotide, modified at its 5'-end with an aldehyde or carboxylic acid, was attached to latex microspheres containing hydrazide residues. Using T4 polynucleotide ligase and an oligonucleotide splint, a single stranded 98mer was efficiently joined to the immobilized synthetic fragment. After impregnation of the latex microspheres with the fluorescent dye, Nile Red and attachment of an aldehyde 16mer, 5 X 10(5) bead-DNA conjugates could be detected with a conventional fluorimeter.

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Selected References

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