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. 1985 Jan 25;13(2):389–399. doi: 10.1093/nar/13.2.389

The nucleotide sequence recognised by the Escherichia coli D type I restriction and modification enzyme.

V Nagaraja, M Stieger, C Nager, S M Hadi, T A Bickle
PMCID: PMC341003  PMID: 2987794

Abstract

A type I restriction endonuclease from a new isolate of Escherichia coli (E. coli E166) has been purified and characterised. The enzyme, EcoD, has a recognition sequence similar in overall structure to the previously determined type I enzyme sequences, an exception being that it is degenerate. The sequence is 5'-T-T-A-N-N-N-N-N-N-N-G-T-C-Y-3' 3'-A-A-T-N-N-N-N-N-N-N-C-A-G-R-5' where Y is a pyrimidine, R is a purine and N can be any nucleotide. The enzyme methylates adenosyl residues in both strands of the DNA that are separated by ten base pairs, suggesting that the enzyme interacts with DNA along one face of the helix making contacts in two successive major grooves.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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