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. 1985 Feb 25;13(4):1283–1302. doi: 10.1093/nar/13.4.1283

An in vitro system for accurate transcription initiation of chloroplast protein genes.

E M Orozco Jr, J E Mullet, N H Chua
PMCID: PMC341072  PMID: 2860636

Abstract

We have developed an homologous in vitro system from spinach chloroplasts that correctly initiates transcription of the plastid genes for the large subunit of ribulose-1,5-bisphosphate carboxylase (rbcL) and the beta subunit of the plastid ATPase (atpB). The transcriptionally active extracts from spinach chloroplasts require circular DNA templates for specific initiation. The RNA polymerase activity is insensitive to rifampicin. The extent of transcription in vitro is a function of the extract:template ratio. The efficiency of the rbcL transcription in vitro is more than one transcript per one hundred templates per hour.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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