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. 1985 Mar 11;13(5):1493–1504. doi: 10.1093/nar/13.5.1493

Nucleic acid (cDNA) and amino acid sequences of the maize endosperm protein glutelin-2.

S Prat, J Cortadas, P Puigdomènech, J Palau
PMCID: PMC341091  PMID: 3839076

Abstract

The cDNA coding for a glutelin-2 protein from maize endosperm has been cloned and the complete amino acid sequence of the protein derived for the first time. An immature maize endosperm cDNA bank was screened for the expression of a beta-lactamase:glutelin-2 (G2) fusion polypeptide by using antibodies against the purified 28 kd G2 protein. A clone corresponding to the 28 kd G2 protein was sequenced and the primary structure of this protein was derived. Five regions can be defined in the protein sequence: an 11 residue N-terminal part, a repeated region formed by eight units of the sequence Pro-Pro-Pro-Val-His-Leu, an alternating Pro-X stretch 21 residues long, a Cys rich domain and a C-terminal part rich in Gln. The protein sequence is preceded by 19 residues which have the characteristics of the signal peptide found in secreted proteins. Unlike zeins, the main maize storage proteins, 28 kd glutelin-2 has several homologous sequences in common with other cereal storage proteins.

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Selected References

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  1. Argos P., Pedersen K., Marks M. D., Larkins B. A. A structural model for maize zein proteins. J Biol Chem. 1982 Sep 10;257(17):9984–9990. [PubMed] [Google Scholar]
  2. Aviv H., Leder P. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose. Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408–1412. doi: 10.1073/pnas.69.6.1408. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bartels D., Thompson R. D. The characterization of cDNA clones coding for wheat storage proteins. Nucleic Acids Res. 1983 May 25;11(10):2961–2977. doi: 10.1093/nar/11.10.2961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bernard O., Gough N. M. Nucleotide sequence of immunoglobulin heavy chain joining segments between translocated VH and mu constant regions genes. Proc Natl Acad Sci U S A. 1980 Jun;77(6):3630–3634. doi: 10.1073/pnas.77.6.3630. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Borgese N., Gaetani S. Site of synthesis of rat liver NADH--cytochrome b5 reductase, an integral membrane protein. FEBS Lett. 1980 Apr 7;112(2):216–220. doi: 10.1016/0014-5793(80)80183-9. [DOI] [PubMed] [Google Scholar]
  6. Burr F. A., Burr B. Three mutations in Zea mays affecting zein accumulation: a comparison of zein polypeptides, in vitro synthesis and processing, mRNA levels, and genomic organization. J Cell Biol. 1982 Jul;94(1):201–206. doi: 10.1083/jcb.94.1.201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Chou P. Y., Fasman G. D. Prediction of protein conformation. Biochemistry. 1974 Jan 15;13(2):222–245. doi: 10.1021/bi00699a002. [DOI] [PubMed] [Google Scholar]
  8. Dretzen G., Bellard M., Sassone-Corsi P., Chambon P. A reliable method for the recovery of DNA fragments from agarose and acrylamide gels. Anal Biochem. 1981 Apr;112(2):295–298. doi: 10.1016/0003-2697(81)90296-7. [DOI] [PubMed] [Google Scholar]
  9. Geraghty D. E., Messing J., Rubenstein I. Sequence analysis and comparison of cDNAs of the zein multigene family . EMBO J. 1982;1(11):1329–1335. doi: 10.1002/j.1460-2075.1982.tb01318.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kasarda D. D., Okita T. W., Bernardin J. E., Baecker P. A., Nimmo C. C., Lew E. J., Dietler M. D., Greene F. C. Nucleic acid (cDNA) and amino acid sequences of alpha-type gliadins from wheat (Triticum aestivum). Proc Natl Acad Sci U S A. 1984 Aug;81(15):4712–4716. doi: 10.1073/pnas.81.15.4712. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kozak M. Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs. Nucleic Acids Res. 1984 Jan 25;12(2):857–872. doi: 10.1093/nar/12.2.857. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  13. Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
  14. Rafalski J. A., Scheets K., Metzler M., Peterson D. M., Hedgcoth C., Söll D. G. Developmentally regulated plant genes: the nucleotide sequence of a wheat gliadin genomic clone. EMBO J. 1984 Jun;3(6):1409–1415. doi: 10.1002/j.1460-2075.1984.tb01985.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Ricciardi R. P., Miller J. S., Roberts B. E. Purification and mapping of specific mRNAs by hybridization-selection and cell-free translation. Proc Natl Acad Sci U S A. 1979 Oct;76(10):4927–4931. doi: 10.1073/pnas.76.10.4927. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Ruiz-Vazquez R., Ruiz-Carillo A. Construction of chimeric plasmids containing histone H5 cDNA from hen erythrocyte. DNA sequence of a fragment derived from the 5' region of H5 mRNA. Nucleic Acids Res. 1982 Mar 25;10(6):2093–2108. doi: 10.1093/nar/10.6.2093. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Taub F., Thompson E. B. An improved method for preparing large arrays of bacterial colonies containing plasmids for hybridization: in situ purification and stable binding of DNA on paper filters. Anal Biochem. 1982 Oct;126(1):222–230. doi: 10.1016/0003-2697(82)90133-6. [DOI] [PubMed] [Google Scholar]
  18. Villa-Komaroff L., Efstratiadis A., Broome S., Lomedico P., Tizard R., Naber S. P., Chick W. L., Gilbert W. A bacterial clone synthesizing proinsulin. Proc Natl Acad Sci U S A. 1978 Aug;75(8):3727–3731. doi: 10.1073/pnas.75.8.3727. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Viotti A., Abildsten D., Pogna N., Sala E., Pirrotta V. Multiplicity and diversity of cloned zein cDNA sequences and their chromosomal localisation. EMBO J. 1982;1(1):53–58. doi: 10.1002/j.1460-2075.1982.tb01123.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Walter P., Gilmore R., Blobel G. Protein translocation across the endoplasmic reticulum. Cell. 1984 Aug;38(1):5–8. doi: 10.1016/0092-8674(84)90520-8. [DOI] [PubMed] [Google Scholar]
  21. Watson M. E. Compilation of published signal sequences. Nucleic Acids Res. 1984 Jul 11;12(13):5145–5164. doi: 10.1093/nar/12.13.5145. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Wickens M. P., Buell G. N., Schimke R. T. Synthesis of double-stranded DNA complementary to lysozyme, ovomucoid, and ovalbumin mRNAs. Optimization for full length second strand synthesis by Escherichia coli DNA polymerase I. J Biol Chem. 1978 Apr 10;253(7):2483–2495. [PubMed] [Google Scholar]
  23. Wienand U., Feix G. Electrophoretic fractionation and translation in vitro of poly(rA)-containing RNA from maize endosperm. Evidence of two mRNAs coding for zein protein. Eur J Biochem. 1978 Dec;92(2):605–611. doi: 10.1111/j.1432-1033.1978.tb12783.x. [DOI] [PubMed] [Google Scholar]

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