Skip to main content
PMC home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1985 Mar 11;13(5):1517–1528. doi: 10.1093/nar/13.5.1517

Two new restriction endonucleases DraII and DraIII from Deinococcus radiophilus.

R Grosskopf, W Wolf, C Kessler
PMCID: PMC341093  PMID: 2987827

Abstract

In addition to recently characterized DraI (1), two new Type II restriction endonucleases, DraII and DraIII, with novel site-specificities were isolated and purified from Deinococcus radiophilus ATCC 27603. DraII and DraIII recognize the hepta- and nonanucleotide sequences (sequence in text) The cleavage sites within both strands are indicated by arrows. The recognition sequences were established by mapping of the cleavage sites on pBR322 (DraII) and fd109 RF DNA (DraIII). The sequence specifities were confirmed by computer-assisted restriction analyses of the generated fragment patterns of the sequenced DNA's of the bacteriophages lambda, phi X174 RF, M13mp8 RF and fd109 RF, the viruses Adeno2 and SV40, and the plasmids pBR322 and pBR328. The cleavage positions within the recognition sequences were determined by sequencing experiments.

Full text

PDF
1525

Images in this article

1522Image
on p.1522
1525Image
on p.1525

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Fiers W., Contreras R., Haegemann G., Rogiers R., Van de Voorde A., Van Heuverswyn H., Van Herreweghe J., Volckaert G., Ysebaert M. Complete nucleotide sequence of SV40 DNA. Nature. 1978 May 11;273(5658):113–120. doi: 10.1038/273113a0. [DOI] [PubMed] [Google Scholar]
  2. Herrmann R., Neugebauer K., Pirkl E., Zentgraf H., Schaller H. Conversion of bacteriophage fd into an efficient single-stranded DNA vector system. Mol Gen Genet. 1980 Jan;177(2):231–242. doi: 10.1007/BF00267434. [DOI] [PubMed] [Google Scholar]
  3. Kessler C., Neumaier P. S., Wolf W. Recognition sequences of restriction endonucleases and methylases--a review. Gene. 1985;33(1):1–102. doi: 10.1016/0378-1119(85)90119-2. [DOI] [PubMed] [Google Scholar]
  4. Maxam A. M., Gilbert W. A new method for sequencing DNA. Proc Natl Acad Sci U S A. 1977 Feb;74(2):560–564. doi: 10.1073/pnas.74.2.560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. McConnell D. J., Searcy D. G., Sutcliffe J. G. A restriction enzyme Tha I from the thermophilic mycoplasma Thermoplasma acidophilum. Nucleic Acids Res. 1978 Jun;5(6):1729–1739. doi: 10.1093/nar/5.6.1729. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Peden K. W. Revised sequence of the tetracycline-resistance gene of pBR322. Gene. 1983 May-Jun;22(2-3):277–280. doi: 10.1016/0378-1119(83)90112-9. [DOI] [PubMed] [Google Scholar]
  7. Purvis I. J., Moseley B. E. Isolation and characterisation of DraI, a type II restriction endonuclease recognising a sequence containing only A:T basepairs, and inhibition of its activity by uv irradiation of substrate DNA. Nucleic Acids Res. 1983 Aug 25;11(16):5467–5474. doi: 10.1093/nar/11.16.5467. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  9. Roberts R. J. Restriction and modification enzymes and their recognition sequences. Nucleic Acids Res. 1984;12 (Suppl):r167–r204. doi: 10.1093/nar/12.suppl.r167. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Sanger F., Air G. M., Barrell B. G., Brown N. L., Coulson A. R., Fiddes C. A., Hutchison C. A., Slocombe P. M., Smith M. Nucleotide sequence of bacteriophage phi X174 DNA. Nature. 1977 Feb 24;265(5596):687–695. doi: 10.1038/265687a0. [DOI] [PubMed] [Google Scholar]
  11. Sanger F., Coulson A. R., Friedmann T., Air G. M., Barrell B. G., Brown N. L., Fiddes J. C., Hutchison C. A., 3rd, Slocombe P. M., Smith M. The nucleotide sequence of bacteriophage phiX174. J Mol Biol. 1978 Oct 25;125(2):225–246. doi: 10.1016/0022-2836(78)90346-7. [DOI] [PubMed] [Google Scholar]
  12. Sanger F., Coulson A. R., Hong G. F., Hill D. F., Petersen G. B. Nucleotide sequence of bacteriophage lambda DNA. J Mol Biol. 1982 Dec 25;162(4):729–773. doi: 10.1016/0022-2836(82)90546-0. [DOI] [PubMed] [Google Scholar]
  13. Shinomiya T., Sato S. A spite specific endonuclease from thermus thermophilus 111, Tth111I. Nucleic Acids Res. 1980 Jan 11;8(1):43–56. doi: 10.1093/nar/8.1.43. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Smith H. O., Nathans D. Letter: A suggested nomenclature for bacterial host modification and restriction systems and their enzymes. J Mol Biol. 1973 Dec 15;81(3):419–423. doi: 10.1016/0022-2836(73)90152-6. [DOI] [PubMed] [Google Scholar]
  15. Soberon X., Covarrubias L., Bolivar F. Construction and characterization of new cloning vehicles. IV. Deletion derivatives of pBR322 and pBR325. Gene. 1980 May;9(3-4):287–305. doi: 10.1016/0378-1119(90)90328-o. [DOI] [PubMed] [Google Scholar]
  16. Timko J., Horwitz A. H., Zelinka J., Wilcox G. Characterization of a site-specific restriction endonuclease from Streptomyces aureofaciens. J Bacteriol. 1981 Feb;145(2):873–877. doi: 10.1128/jb.145.2.873-877.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Van Heuverswyn H., Fiers W. Nucleotide sequence of the Hind-C fragment of simian virus 40 DNA. Comparison of the 5'-untranslated region of wild-type virus and of some deletion Mutants. Eur J Biochem. 1979 Oct;100(1):51–60. doi: 10.1111/j.1432-1033.1979.tb02032.x. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES