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. 1985 Apr 11;13(7):2583–2601. doi: 10.1093/nar/13.7.2583

Novel non-suppressing mutants of Escherichia coli tRNATyr su+3.

P Furdon, S Altman
PMCID: PMC341177  PMID: 3889847

Abstract

Several addition and deletion mutations were constructed in the region of the gene for Escherichia coli tRNATyr su+3 corresponding to the dihydrouracil loop of the mature tRNA. None of these resulting mutants had detectable suppressor function compared to the parent gene yet some directed the synthesis of mature tRNA. These latter mutants may affect the ability of the tRNA to be aminoacylated or to interact with the translational machinery on the ribosome.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abelson J. N., Gefter M. L., Barnett L., Landy A., Russell R. L., Smith J. D. Mutant tyrosine transfer ribonucleic acids. J Mol Biol. 1970 Jan 14;47(1):15–28. doi: 10.1016/0022-2836(70)90398-0. [DOI] [PubMed] [Google Scholar]
  2. Benzer S., Champe S. P. AMBIVALENT rII MUTANTS OF PHAGE T4. Proc Natl Acad Sci U S A. 1961 Jul;47(7):1025–1038. doi: 10.1073/pnas.47.7.1025. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Boyer H. W., Roulland-Dussoix D. A complementation analysis of the restriction and modification of DNA in Escherichia coli. J Mol Biol. 1969 May 14;41(3):459–472. doi: 10.1016/0022-2836(69)90288-5. [DOI] [PubMed] [Google Scholar]
  4. Celis J. E., Piper P. W. Compilation of mutant suppressor tRNA sequences. Nucleic Acids Res. 1982 Jan 22;10(2):r83–r91. doi: 10.1093/nar/10.2.762-b. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Folk W. R., Berg P. Isolation and partial characterization of Escherichia coli mutants with altered glycyl transfer ribonucleic acid synthetases. J Bacteriol. 1970 Apr;102(1):193–203. doi: 10.1128/jb.102.1.193-203.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Furdon P. J., Guerrier-Takada C., Altman S. A G43 to U43 mutation in E. coli tRNAtyrsu3+ which affects processing by RNase P. Nucleic Acids Res. 1983 Mar 11;11(5):1491–1505. doi: 10.1093/nar/11.5.1491. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Holmes D. S., Quigley M. A rapid boiling method for the preparation of bacterial plasmids. Anal Biochem. 1981 Jun;114(1):193–197. doi: 10.1016/0003-2697(81)90473-5. [DOI] [PubMed] [Google Scholar]
  8. Hooper M. L., Russell R. L., Smith J. D. Mischarging in mutant tyrosine transfer RNAs. FEBS Lett. 1972 Apr 15;22(1):149–155. doi: 10.1016/0014-5793(72)80241-2. [DOI] [PubMed] [Google Scholar]
  9. Hörz W., Zachau H. G. Complexes of aminoacyl-tRNA synthetases with tRNAs as studies by partial nuclease digestion. Eur J Biochem. 1973 Jan 3;32(1):1–14. doi: 10.1111/j.1432-1033.1973.tb02571.x. [DOI] [PubMed] [Google Scholar]
  10. Kaplan S., Stretton A. O., Brenner S. Amber suppressors: efficiency of chain propagation and suppressor specific amino acids. J Mol Biol. 1965 Dec;14(2):528–533. doi: 10.1016/s0022-2836(65)80202-9. [DOI] [PubMed] [Google Scholar]
  11. Queen C. L., Korn L. J. Computer analysis of nucleic acids and proteins. Methods Enzymol. 1980;65(1):595–609. doi: 10.1016/s0076-6879(80)65062-9. [DOI] [PubMed] [Google Scholar]
  12. Rich A., RajBhandary U. L. Transfer RNA: molecular structure, sequence, and properties. Annu Rev Biochem. 1976;45:805–860. doi: 10.1146/annurev.bi.45.070176.004105. [DOI] [PubMed] [Google Scholar]
  13. Russell R. L., Abelson J. N., Landy A., Gefter M. L., Brenner S., Smith J. D. Duplicate genes for tyrosine transfer RNA in Escherichia coli. J Mol Biol. 1970 Jan 14;47(1):1–13. doi: 10.1016/0022-2836(70)90397-9. [DOI] [PubMed] [Google Scholar]
  14. Schedl P., Primakoff P. Mutants of Escherichia coli thermosensitive for the synthesis of transfer RNA. Proc Natl Acad Sci U S A. 1973 Jul;70(7):2091–2095. doi: 10.1073/pnas.70.7.2091. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Smith H. O., Birnstiel M. L. A simple method for DNA restriction site mapping. Nucleic Acids Res. 1976 Sep;3(9):2387–2398. doi: 10.1093/nar/3.9.2387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Smith J. D., Barnett L., Brenner S., Russell R. L. More mutant tyrosine transfer ribonucleic acids. J Mol Biol. 1970 Nov 28;54(1):1–14. doi: 10.1016/0022-2836(70)90442-0. [DOI] [PubMed] [Google Scholar]
  17. Weiner A. M. An abundant cytoplasmic 7S RNA is complementary to the dominant interspersed middle repetitive DNA sequence family in the human genome. Cell. 1980 Nov;22(1 Pt 1):209–218. doi: 10.1016/0092-8674(80)90169-5. [DOI] [PubMed] [Google Scholar]
  18. Yarus M., Breeden L. Mutants of Su+7 tRNA include a functional tRNA with an altered T pseudo uracil CG sequence. Cell. 1981 Sep;25(3):815–823. doi: 10.1016/0092-8674(81)90189-6. [DOI] [PubMed] [Google Scholar]
  19. de Bruijn M. H., Klug A. A model for the tertiary structure of mammalian mitochondrial transfer RNAs lacking the entire 'dihydrouridine' loop and stem. EMBO J. 1983;2(8):1309–1321. doi: 10.1002/j.1460-2075.1983.tb01586.x. [DOI] [PMC free article] [PubMed] [Google Scholar]

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