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. 1985 Apr 25;13(8):2773–2788. doi: 10.1093/nar/13.8.2773

Cloning and nucleotide sequence of the tzs gene from Agrobacterium tumefaciens strain T37.

D E Akiyoshi, D A Regier, G Jen, M P Gordon
PMCID: PMC341193  PMID: 2987868

Abstract

The trans-zeatin secretion locus (tzs), from the nopaline Ti plasmid of Agrobacterium tumefaciens strain T37, was cloned and the nucleotide sequence determined. This gene is located in the virulence region of pTiT37. The tzs gene is responsible for the secretion of trans-zeatin into bacterial culture medium and in addition has the cytokinin biosynthetic activity, dimethylallylpyrophosphate:AMP dimethylallyltransferase. Sequence analysis showed an open reading frame of 729 nucleotides, capable of encoding a protein of 27,545 daltons. A single new labelled protein of 27,200 daltons was detected in Escherichia coli maxicells expressing the cloned tzs gene. Significant sequence homology was observed between the tzs and the published tmr sequence from pTiT37.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Akiyoshi D. E., Klee H., Amasino R. M., Nester E. W., Gordon M. P. T-DNA of Agrobacterium tumefaciens encodes an enzyme of cytokinin biosynthesis. Proc Natl Acad Sci U S A. 1984 Oct;81(19):5994–5998. doi: 10.1073/pnas.81.19.5994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barry G. F., Rogers S. G., Fraley R. T., Brand L. Identification of a cloned cytokinin biosynthetic gene. Proc Natl Acad Sci U S A. 1984 Aug;81(15):4776–4780. doi: 10.1073/pnas.81.15.4776. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bevan M. W., Chilton M. D. T-DNA of the Agrobacterium Ti and Ri plasmids. Annu Rev Genet. 1982;16:357–384. doi: 10.1146/annurev.ge.16.120182.002041. [DOI] [PubMed] [Google Scholar]
  4. Bevan M., Barnes W. M., Chilton M. D. Structure and transcription of the nopaline synthase gene region of T-DNA. Nucleic Acids Res. 1983 Jan 25;11(2):369–385. doi: 10.1093/nar/11.2.369. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Biggin M. D., Gibson T. J., Hong G. F. Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination. Proc Natl Acad Sci U S A. 1983 Jul;80(13):3963–3965. doi: 10.1073/pnas.80.13.3963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Birnboim H. C., Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979 Nov 24;7(6):1513–1523. doi: 10.1093/nar/7.6.1513. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Boyer H. W., Roulland-Dussoix D. A complementation analysis of the restriction and modification of DNA in Escherichia coli. J Mol Biol. 1969 May 14;41(3):459–472. doi: 10.1016/0022-2836(69)90288-5. [DOI] [PubMed] [Google Scholar]
  8. Braun A. C. A Physiological Basis for Autonomous Growth of the Crown-Gall Tumor Cell. Proc Natl Acad Sci U S A. 1958 Apr;44(4):344–349. doi: 10.1073/pnas.44.4.344. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Buchholz W. G., Thomashow M. F. Comparison of T-DNA oncogene complements of Agrobacterium tumefaciens tumor-inducing plasmids with limited and wide host ranges. J Bacteriol. 1984 Oct;160(1):319–326. doi: 10.1128/jb.160.1.319-326.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Chapman R. W., Morris R. O., Zaerr J. B. Occurrence of trans-ribosylzeatin in Agrobacterium tumefaciens tRNA. Nature. 1976 Jul 8;262(5564):153–154. doi: 10.1038/262153a0. [DOI] [PubMed] [Google Scholar]
  11. Cherayil J. D., Lipsett M. N. Zeatin ribonucleosides in the transfer ribonucleic acid of Rhizobium leguminosarum, Agrobacterium tumefaciens, Corynebacterium fascians, and Erwinia amylovora. J Bacteriol. 1977 Sep;131(3):741–744. doi: 10.1128/jb.131.3.741-744.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Chilton M. D., Currier T. C., Farrand S. K., Bendich A. J., Gordon M. P., Nester E. W. Agrobacterium tumefaciens DNA and PS8 bacteriophage DNA not detected in crown gall tumors. Proc Natl Acad Sci U S A. 1974 Sep;71(9):3672–3676. doi: 10.1073/pnas.71.9.3672. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. De Greve H., Dhaese P., Seurinck J., Lemmers M., Van Montagu M., Schell J. Nucleotide sequence and transcript map of the Agrobacterium tumefaciens Ti plasmid-encoded octopine synthase gene. J Mol Appl Genet. 1982;1(6):499–511. [PubMed] [Google Scholar]
  14. Garfinkel D. J., Nester E. W. Agrobacterium tumefaciens mutants affected in crown gall tumorigenesis and octopine catabolism. J Bacteriol. 1980 Nov;144(2):732–743. doi: 10.1128/jb.144.2.732-743.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Garfinkel D. J., Simpson R. B., Ream L. W., White F. F., Gordon M. P., Nester E. W. Genetic analysis of crown gall: fine structure map of the T-DNA by site-directed mutagenesis. Cell. 1981 Nov;27(1 Pt 2):143–153. doi: 10.1016/0092-8674(81)90368-8. [DOI] [PubMed] [Google Scholar]
  16. Goldberg S. B., Flick J. S., Rogers S. G. Nucleotide sequence of the tmr locus of Agrobacterium tumefaciens pTi T37 T-DNA. Nucleic Acids Res. 1984 Jun 11;12(11):4665–4677. doi: 10.1093/nar/12.11.4665. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Guo L. H., Wu R. New rapid methods for DNA sequencing based in exonuclease III digestion followed by repair synthesis. Nucleic Acids Res. 1982 Mar 25;10(6):2065–2084. doi: 10.1093/nar/10.6.2065. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Heidekamp F., Dirkse W. G., Hille J., van Ormondt H. Nucleotide sequence of the Agrobacterium tumefaciens octopine Ti plasmid-encoded tmr gene. Nucleic Acids Res. 1983 Sep 24;11(18):6211–6223. doi: 10.1093/nar/11.18.6211. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Hoekema A., de Pater B. S., Fellinger A. J., Hooykaas P. J., Schilperoort R. A. The limited host range of an Agrobacterium tumefaciens strain extended by a cytokinin gene from a wide host range T-region. EMBO J. 1984 Dec 20;3(13):3043–3047. doi: 10.1002/j.1460-2075.1984.tb02255.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Hohn B., Collins J. A small cosmid for efficient cloning of large DNA fragments. Gene. 1980 Nov;11(3-4):291–298. doi: 10.1016/0378-1119(80)90069-4. [DOI] [PubMed] [Google Scholar]
  21. Hooykaas P. J., Schilperoort R. A. The molecular genetics of crown gall tumorigenesis. Adv Genet. 1984;22:209–283. doi: 10.1016/s0065-2660(08)60041-3. [DOI] [PubMed] [Google Scholar]
  22. Joos H., Inzé D., Caplan A., Sormann M., Van Montagu M., Schell J. Genetic analysis of T-DNA transcripts in nopaline crown galls. Cell. 1983 Apr;32(4):1057–1067. doi: 10.1016/0092-8674(83)90290-8. [DOI] [PubMed] [Google Scholar]
  23. KAPER J. M., VELDSTRA H. On the metabolism of tryptophan by Agrobacterium tumefaciens. Biochim Biophys Acta. 1958 Nov;30(2):401–420. doi: 10.1016/0006-3002(58)90065-9. [DOI] [PubMed] [Google Scholar]
  24. Kaiss-Chapman R. W., Morris R. O. Trans-zeatin in culture filtrates of Agrobacterium tumefaciens. Biochem Biophys Res Commun. 1976 May 23;76(2):453–459. doi: 10.1016/0006-291x(77)90746-x. [DOI] [PubMed] [Google Scholar]
  25. Kao J. C., Perry K. L., Kado C. I. Indoleacetic acid complementation and its relation to host range specifying genes on the Ti plasmid of Agrobacterium tumefaciens. Mol Gen Genet. 1982;188(3):425–432. doi: 10.1007/BF00330044. [DOI] [PubMed] [Google Scholar]
  26. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  27. Leemans J., Deblaere R., Willmitzer L., De Greve H., Hernalsteens J. P., Van Montagu M., Schell J. Genetic Identification of functions of TL-DNA transcripts in octopine crown galls. EMBO J. 1982;1(1):147–152. doi: 10.1002/j.1460-2075.1982.tb01138.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Lichtenstein C., Klee H., Montoya A., Garfinkel D., Fuller S., Flores C., Nester E., Gordon M. Nucleotide sequence and transcript mapping of the tmr gene of the pTiA6NC octopine Ti-plasmid: a bacterial gene involved in plant tumorigenesis. J Mol Appl Genet. 1984;2(4):354–362. [PubMed] [Google Scholar]
  29. Liu S. T., Kado C. I. Indoleacetic acid production: a plasmid function of Agrobacterium tumefaciens C58. Biochem Biophys Res Commun. 1979 Sep 12;90(1):171–178. doi: 10.1016/0006-291x(79)91605-x. [DOI] [PubMed] [Google Scholar]
  30. Liu S. T., Perry K. L., Schardl C. L., Kado C. I. Agrobacterium Ti plasmid indoleacetic acid gene is required for crown gall oncogenesis. Proc Natl Acad Sci U S A. 1982 May;79(9):2812–2816. doi: 10.1073/pnas.79.9.2812. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. McCloskey J. A., Hashizume T., Basile B., Ohno Y., Sonoki S. Occurrence and levels of cis-and trans-zeatin ribosides in the culture medium of a virulent strain of Agrobacterium tumefaciens. FEBS Lett. 1980 Feb 25;111(1):181–183. doi: 10.1016/0014-5793(80)80788-5. [DOI] [PubMed] [Google Scholar]
  32. Messing J., Crea R., Seeburg P. H. A system for shotgun DNA sequencing. Nucleic Acids Res. 1981 Jan 24;9(2):309–321. doi: 10.1093/nar/9.2.309. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Murai N., Kemp J. D. Octopine synthase mRNA isolated from sunflower crown gall callus is homologous to the Ti plasmid of Agrobacterium tumefaciens. Proc Natl Acad Sci U S A. 1982 Jan;79(1):86–90. doi: 10.1073/pnas.79.1.86. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Murai N., Skoog F., Doyle M. E., Hanson R. S. Relationships between cytokinin production, presence of plasmids, and fasciation caused by strains of Corynebacterium fascians. Proc Natl Acad Sci U S A. 1980 Jan;77(1):619–623. doi: 10.1073/pnas.77.1.619. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Nester E. W., Kosuge T. Plasmids specifying plant hyperplasias. Annu Rev Microbiol. 1981;35:531–565. doi: 10.1146/annurev.mi.35.100181.002531. [DOI] [PubMed] [Google Scholar]
  36. Norrander J., Kempe T., Messing J. Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis. Gene. 1983 Dec;26(1):101–106. doi: 10.1016/0378-1119(83)90040-9. [DOI] [PubMed] [Google Scholar]
  37. Ooms G., Hooykaas P. J., Moolenaar G., Schilperoort R. A. Grown gall plant tumors of abnormal morphology, induced by Agrobacterium tumefaciens carrying mutated octopine Ti plasmids; analysis of T-DNA functions. Gene. 1981 Jun-Jul;14(1-2):33–50. doi: 10.1016/0378-1119(81)90146-3. [DOI] [PubMed] [Google Scholar]
  38. Regier D. A., Morris R. O. Secretion of trans-zeatin by Agrobacterium tumefaciens: a function determined by the nopaline Ti plasmid. Biochem Biophys Res Commun. 1982 Feb 26;104(4):1560–1566. doi: 10.1016/0006-291x(82)91429-2. [DOI] [PubMed] [Google Scholar]
  39. Rosenberg M., Court D. Regulatory sequences involved in the promotion and termination of RNA transcription. Annu Rev Genet. 1979;13:319–353. doi: 10.1146/annurev.ge.13.120179.001535. [DOI] [PubMed] [Google Scholar]
  40. Sancar A., Hack A. M., Rupp W. D. Simple method for identification of plasmid-coded proteins. J Bacteriol. 1979 Jan;137(1):692–693. doi: 10.1128/jb.137.1.692-693.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  41. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  42. Schröder G., Waffenschmidt S., Weiler E. W., Schröder J. The T-region of Ti plasmids codes for an enzyme synthesizing indole-3-acetic acid. Eur J Biochem. 1984 Jan 16;138(2):387–391. doi: 10.1111/j.1432-1033.1984.tb07927.x. [DOI] [PubMed] [Google Scholar]
  43. Sciaky D., Montoya A. L., Chilton M. D. Fingerprints of Agrobacterium Ti plasmids. Plasmid. 1978 Feb;1(2):238–253. doi: 10.1016/0147-619x(78)90042-2. [DOI] [PubMed] [Google Scholar]
  44. Shine J., Dalgarno L. The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites. Proc Natl Acad Sci U S A. 1974 Apr;71(4):1342–1346. doi: 10.1073/pnas.71.4.1342. [DOI] [PMC free article] [PubMed] [Google Scholar]
  45. Sonoki S., Ohno Y., Kijima N., Hishiyama T., Saito H., Sugiyama T., Hashizume T. Relationship between adenylate cytokinin production and Ti plasmid of Agrobacterium tumefaciens. Nucleic Acids Symp Ser. 1983;(12):111–114. [PubMed] [Google Scholar]

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