Skip to main content
PMC home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1985 Apr 25;13(8):2959–2977. doi: 10.1093/nar/13.8.2959

Chemical synthesis of a gene for somatomedin C.

B S Sproat, M J Gait
PMCID: PMC341207  PMID: 2987870

Abstract

A synthetic gene for somatomedin C, a human growth factor, has been assembled by a single ligation of 23 oligodeoxyribonucleotides, which were chemically synthesized by an improved solid phase phosphotriester method.

Full text

PDF
2974

Images in this article

2966Image
on p.2966
2967Image
on p.2967
2968Image
on p.2968

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Biggin M. D., Gibson T. J., Hong G. F. Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination. Proc Natl Acad Sci U S A. 1983 Jul;80(13):3963–3965. doi: 10.1073/pnas.80.13.3963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Daughaday W. H., Hall K., Raben M. S., Salmon W. D., Jr, van den Brande J. L., van Wyk J. J. Somatomedin: proposed designation for sulphation factor. Nature. 1972 Jan 14;235(5333):107–107. doi: 10.1038/235107a0. [DOI] [PubMed] [Google Scholar]
  3. Frank R., Heikens W., Heisterberg-Moutsis G., Blöcker H. A new general approach for the simultaneous chemical synthesis of large numbers of oligonucleotides: segmental solid supports. Nucleic Acids Res. 1983 Jul 11;11(13):4365–4377. doi: 10.1093/nar/11.13.4365. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Froesch E. R., Zapf J., Meuli C., Mäder M., Waldvogel M., Kaufmann U., Morell B. Biological properties of NSILA-S. Adv Metab Disord. 1975;8:211–235. doi: 10.1016/b978-0-12-027308-9.50021-4. [DOI] [PubMed] [Google Scholar]
  5. Gouy M., Gautier C. Codon usage in bacteria: correlation with gene expressivity. Nucleic Acids Res. 1982 Nov 25;10(22):7055–7074. doi: 10.1093/nar/10.22.7055. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hanahan D. Studies on transformation of Escherichia coli with plasmids. J Mol Biol. 1983 Jun 5;166(4):557–580. doi: 10.1016/s0022-2836(83)80284-8. [DOI] [PubMed] [Google Scholar]
  7. Herington A. C., Cornell H. J., Kuffer A. D. Recent advances in the biochemistry and physiology of the insulin-like growth factor/somatomedin family. Int J Biochem. 1983;15(10):1201–1210. doi: 10.1016/0020-711x(83)90208-2. [DOI] [PubMed] [Google Scholar]
  8. Ikemura T. Correlation between the abundance of Escherichia coli transfer RNAs and the occurrence of the respective codons in its protein genes. J Mol Biol. 1981 Feb 15;146(1):1–21. doi: 10.1016/0022-2836(81)90363-6. [DOI] [PubMed] [Google Scholar]
  9. Ikemura T. Correlation between the abundance of Escherichia coli transfer RNAs and the occurrence of the respective codons in its protein genes: a proposal for a synonymous codon choice that is optimal for the E. coli translational system. J Mol Biol. 1981 Sep 25;151(3):389–409. doi: 10.1016/0022-2836(81)90003-6. [DOI] [PubMed] [Google Scholar]
  10. Klapper D. G., Svoboda M. E., Van Wyk J. J. Sequence analysis of somatomedin-C: confirmation of identity with insulin-like growth factor I. Endocrinology. 1983 Jun;112(6):2215–2217. doi: 10.1210/endo-112-6-2215. [DOI] [PubMed] [Google Scholar]
  11. Laron Z. Laron-type dwarfism (hereditary somatomedin deficiency): a review. Ergeb Inn Med Kinderheilkd. 1984;51:117–150. doi: 10.1007/978-3-642-69070-9_3. [DOI] [PubMed] [Google Scholar]
  12. Li C. H., Yamashiro D., Gospodarowicz D., Kaplan S. L., Van Vliet G. Total synthesis of insulin-like growth factor I (somatomedin C). Proc Natl Acad Sci U S A. 1983 Apr;80(8):2216–2220. doi: 10.1073/pnas.80.8.2216. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Liberti J. P., Miller M. S. The purification and partial characterization of human somatomedin C. J Biol Chem. 1980 Feb 10;255(3):1023–1029. [PubMed] [Google Scholar]
  14. Mandel M., Higa A. Calcium-dependent bacteriophage DNA infection. J Mol Biol. 1970 Oct 14;53(1):159–162. doi: 10.1016/0022-2836(70)90051-3. [DOI] [PubMed] [Google Scholar]
  15. Matthes H. W., Zenke W. M., Grundström T., Staub A., Wintzerith M., Chambon P. Simultaneous rapid chemical synthesis of over one hundred oligonucleotides on a microscale. EMBO J. 1984 Apr;3(4):801–805. doi: 10.1002/j.1460-2075.1984.tb01888.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Ohtsuka E., Taniyama Y., Iwai S., Kitano K., Miyamoto S., Ohgi T., Sakuragawa Y., Fujiyama K., Ikari T., Kobayashi M. The synthesis of human growth hormone gene. Nucleic Acids Symp Ser. 1983;(12):79–82. [PubMed] [Google Scholar]
  17. Rinderknecht E., Humbel R. E. Polypeptides with nonsuppressible insulin-like and cell-growth promoting activities in human serum: isolation, chemical characterization, and some biological properties of forms I and II. Proc Natl Acad Sci U S A. 1976 Jul;73(7):2365–2369. doi: 10.1073/pnas.73.7.2365. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Rinderknecht E., Humbel R. E. The amino acid sequence of human insulin-like growth factor I and its structural homology with proinsulin. J Biol Chem. 1978 Apr 25;253(8):2769–2776. [PubMed] [Google Scholar]
  19. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Sproat B. S., Brown D. M. A new linkage for solid phase synthesis of oligodeoxyribonucleotides. Nucleic Acids Res. 1985 Apr 25;13(8):2979–2987. doi: 10.1093/nar/13.8.2979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Svoboda M. E., Van Wyk J. J., Klapper D. G., Fellows R. E., Grissom F. E., Schlueter R. J. Purification of somatomedin-C from human plasma: chemical and biological properties, partial sequence analysis, and relationship to other somatomedins. Biochemistry. 1980 Feb 19;19(4):790–797. doi: 10.1021/bi00545a027. [DOI] [PubMed] [Google Scholar]
  22. Urdea M. S., Merryweather J. P., Mullenbach G. T., Coit D., Heberlein U., Valenzuela P., Barr P. J. Chemical synthesis of a gene for human epidermal growth factor urogastrone and its expression in yeast. Proc Natl Acad Sci U S A. 1983 Dec;80(24):7461–7465. doi: 10.1073/pnas.80.24.7461. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Van Wyk J. J., Russell W. E., Li C. H. Synthetic somatomedin C: comparison with natural hormone isolated from human plasma. Proc Natl Acad Sci U S A. 1984 Feb;81(3):740–742. doi: 10.1073/pnas.81.3.740. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Van Wyk J. J., Underwood L. E., Hintz R. L., Clemmons D. R., Voina S. J., Weaver R. P. The somatomedins: a family of insulinlike hormones under growth hormone control. Recent Prog Horm Res. 1974;30(0):259–318. [PubMed] [Google Scholar]
  25. Vogelstein B., Gillespie D. Preparative and analytical purification of DNA from agarose. Proc Natl Acad Sci U S A. 1979 Feb;76(2):615–619. doi: 10.1073/pnas.76.2.615. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES