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. 1985 May 10;13(9):3357–3369. doi: 10.1093/nar/13.9.3357

Hormonal regulation of transcription of rDNA: glucocorticoid effects upon initiation and elongation in vitro.

A H Cavanaugh, E A Thompson Jr
PMCID: PMC341240  PMID: 4000974

Abstract

Various parameters of transcription of cloned mouse rDNA have been examined using extracts from control P1798 cells and from cells treated 24 h with 0.1 microM dexamethasone. Highly purified RNA polymerase I from either source catalyzes nucleotidyl transfer (elongation) at a rate of approximately 30 nucleotides/sec. Extracts from hormone-treated cells are capable of forming stable, preinitiation complexes. The rates of stable complex formation are the same in extracts from control and hormone-treated cells. Nevertheless, initiation of transcription does not occur in extracts from hormone-treated cells. Initiation in such extracts may be restored by the addition of a partially purified RNA polymerase I initiation factor, designated TFIC. The data indicate that initiation by RNA polymerase I is a multi-step process. The first step involves the formation of stable, preinitiation complexes, as demonstrated by a number of groups. Initiation, per se, requires an additional protein, TFIC. Glucocorticoids and perhaps other mitogenic agents regulate transcription of rDNA by influencing the amount or activity of TFIC.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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