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. 1986 Dec 22;14(24):9989–9999. doi: 10.1093/nar/14.24.9989

Nucleotide sequence and organization of dnaB gene and neighbouring genes on the Bacillus subtilis chromosome.

N Ogasawara, S Moriya, P G Mazza, H Yoshikawa
PMCID: PMC341350  PMID: 3027671

Abstract

A region of the Bacillus subtilis chromosome containing dnaB, a gene essential for the initiation of chromosomal replication in B. subtilis, was cloned and nucleotide sequence of some 5000bp determined. The region consists of 4 open reading frames (ORFs) possibly comprising a single transcriptional unit. Two dnaB mutations, dnaB27 and dnaB19 were located within the first ORF which would give rise to a protein of 472 amino acids. The dnaB27 mutation involves codon at the position of 122 (replacement of Asp by Asn) close to a DNA binding domain and the dnaB19 codon 379 (replacement of Ala by Thr) close to a region rich in charged amino acids which may be alpha helical. The third ORF in the same transcriptional unit would produce a hydrophobic protein which might be involved in the DNA-membrane binding function of dnaB gene. No homologous Escherichia coli genes have been found.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chou P. Y., Fasman G. D. Prediction of the secondary structure of proteins from their amino acid sequence. Adv Enzymol Relat Areas Mol Biol. 1978;47:45–148. doi: 10.1002/9780470122921.ch2. [DOI] [PubMed] [Google Scholar]
  2. Contente S., Dubnau D. Characterization of plasmid transformation in Bacillus subtilis: kinetic properties and the effect of DNA conformation. Mol Gen Genet. 1979 Jan 2;167(3):251–258. doi: 10.1007/BF00267416. [DOI] [PubMed] [Google Scholar]
  3. Ferrari F. A., Nguyen A., Lang D., Hoch J. A. Construction and properties of an integrable plasmid for Bacillus subtilis. J Bacteriol. 1983 Jun;154(3):1513–1515. doi: 10.1128/jb.154.3.1513-1515.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gill D. R., Hatfull G. F., Salmond G. P. A new cell division operon in Escherichia coli. Mol Gen Genet. 1986 Oct;205(1):134–145. doi: 10.1007/BF02428043. [DOI] [PubMed] [Google Scholar]
  5. Gitt M. A., Wang L. F., Doi R. H. A strong sequence homology exists between the major RNA polymerase sigma factors of Bacillus subtilis and Escherichia coli. J Biol Chem. 1985 Jun 25;260(12):7178–7185. [PubMed] [Google Scholar]
  6. Imada S., Carroll L. E., Sueoka N. Genetic mapping of a group of temperature-sensitive dna initiation mutants in Bacillus subtilis. Genetics. 1980 Apr;94(4):809–823. doi: 10.1093/genetics/94.4.809. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Karamata D., Gross J. D. Isolation and genetic analysis of temperature-sensitive mutants of B. subtilis defective in DNA synthesis. Mol Gen Genet. 1970;108(3):277–287. doi: 10.1007/BF00283358. [DOI] [PubMed] [Google Scholar]
  8. Laurent S. J., Vannier F. S. Temperature-sensitive initiation of chromosome replication in a mutant of Bacillus subtilis. J Bacteriol. 1973 May;114(2):474–484. doi: 10.1128/jb.114.2.474-484.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Mayaux J. F., Fayat G., Fromant M., Springer M., Grunberg-Manago M., Blanquet S. Structural and transcriptional evidence for related thrS and infC expression. Proc Natl Acad Sci U S A. 1983 Oct;80(20):6152–6156. doi: 10.1073/pnas.80.20.6152. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Mendelson N. H., Gross J. D. Characterization of a temperature-sensitive mutant of Bacillus subtilis defective in deoxyribonucleic acid replication. J Bacteriol. 1967 Nov;94(5):1603–1608. doi: 10.1128/jb.94.5.1603-1608.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Moriya S., Ogasawara N., Yoshikawa H. Structure and function of the region of the replication origin of the Bacillus subtilis chromosome. III. Nucleotide sequence of some 10,000 base pairs in the origin region. Nucleic Acids Res. 1985 Apr 11;13(7):2251–2265. doi: 10.1093/nar/13.7.2251. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Murakami S., Inuzuka N., Yamaguchi M., Yamaguchi K., Yoshikawa H. Initiation of DNA replication in Bacillus subtilis. III. Analysis of molecular events involved in the initiation using a temperature-sensitive dna mutant. J Mol Biol. 1976 Dec 25;108(4):683–704. doi: 10.1016/s0022-2836(76)80112-x. [DOI] [PubMed] [Google Scholar]
  13. Murakami S., Yoshikawa H. Gene that controls initiation of chromosome replication and prophage induction in Bacillus subtilis. Nature. 1976 Jan 22;259(5540):215–218. doi: 10.1038/259215a0. [DOI] [PubMed] [Google Scholar]
  14. Ogasawara N., Moriya S., von Meyenburg K., Hansen F. G., Yoshikawa H. Conservation of genes and their organization in the chromosomal replication origin region of Bacillus subtilis and Escherichia coli. EMBO J. 1985 Dec 1;4(12):3345–3350. doi: 10.1002/j.1460-2075.1985.tb04087.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Ogasawara N., Seiki M., Yoshikawa H. Effect of novobiocin on initiation of DNA replication in Bacillus subtilis. Nature. 1979 Oct 25;281(5733):702–704. doi: 10.1038/281702a0. [DOI] [PubMed] [Google Scholar]
  16. Pabo C. O., Sauer R. T. Protein-DNA recognition. Annu Rev Biochem. 1984;53:293–321. doi: 10.1146/annurev.bi.53.070184.001453. [DOI] [PubMed] [Google Scholar]
  17. White K., Sueoka N. Temperature-sensitive DNA synthesis mutants of Bacillus subtilis--appendix: theory of density transfer for symmetric chromosome replication. Genetics. 1973 Feb;73(2):185–214. doi: 10.1093/genetics/73.2.185. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Winston S., Sueoka N. DNA-membrane association is necessary for initiation of chromosomal and plasmid replication in Bacillus subtilis. Proc Natl Acad Sci U S A. 1980 May;77(5):2834–2838. doi: 10.1073/pnas.77.5.2834. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Yoshikawa H. The initiation of DNA replication in Bacillus subtilis. Proc Natl Acad Sci U S A. 1967 Jul;58(1):312–319. doi: 10.1073/pnas.58.1.312. [DOI] [PMC free article] [PubMed] [Google Scholar]

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