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. 1978 Jan;5(1):297–303. doi: 10.1093/nar/5.1.297

Affinity adsorbents consisting of nucleic acids immobilized via bisoxirane activated polysaccharides.

H Potuzak, P D Dean
PMCID: PMC341978  PMID: 25416

Abstract

An easy and efficient procedure for the immobilization of polynucleotide ligands to bisoxirane activated insoluble polysaccharides has been elaborated and is described in this paper. The resulting materials have been applied to the chromatography of DNA polymerase I, and RNA polymerase from E.coli. Because of their extraordinary stability to temperature, formamide, and alkaline conditions they seem to be particularly useful adsorbents for nucleic acid hybridization.

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Selected References

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  1. Alberts B. M., Amodio F. J., Jenkins M., Gutmann E. D., Ferris F. L. Studies with DNA-cellulose chromatography. I. DNA-binding proteins from Escherichia coli. Cold Spring Harb Symp Quant Biol. 1968;33:289–305. doi: 10.1101/sqb.1968.033.01.033. [DOI] [PubMed] [Google Scholar]
  2. Arndt-Jovin D. J., Jovin T. M., Bähr W., Frischauf A. M., Marquardt M. Covalent attachment of DNA to agarose. Improved synthesis and use in affinity chromatography. Eur J Biochem. 1975 Jun;54(2):411–418. doi: 10.1111/j.1432-1033.1975.tb04151.x. [DOI] [PubMed] [Google Scholar]
  3. Fedoroff N. V., Zinder N. D. Structure of the poly(G) polymerase component of the bacteriophage f2 replicase. Proc Natl Acad Sci U S A. 1971 Aug;68(8):1838–1843. doi: 10.1073/pnas.68.8.1838. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Haselkorn R., Rouvière-Yaniv J. Cyanobacterial DNA-binding protein related to Escherichia coli HU. Proc Natl Acad Sci U S A. 1976 Jun;73(6):1917–1920. doi: 10.1073/pnas.73.6.1917. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Jovin T. M., Englund P. T., Bertsch L. L. Enzymatic synthesis of deoxyribonucleic acid. XXVI. Physical and chemical studies of a homogeneous deoxyribonucleic acid polymerase. J Biol Chem. 1969 Jun 10;244(11):2996–3008. [PubMed] [Google Scholar]
  6. Levinson A., Levine A. J., Anderson S., Osborn M., Rosenwirth B., Weber K. The relationship between group C adenovirus tumor antigen and the adenovirus single-strand DNA-binding protein. Cell. 1976 Apr;7(4):575–584. doi: 10.1016/0092-8674(76)90208-7. [DOI] [PubMed] [Google Scholar]
  7. Litman R. M. A deoxyribonucleic acid polymerase from Micrococcus luteus (Micrococcus lysodeikticus) isolated on deoxyribonucleic acid-cellulose. J Biol Chem. 1968 Dec 10;243(23):6222–6233. [PubMed] [Google Scholar]
  8. Matsukage A., Sivarajan M., Wilson S. H. Studies on DNA alpha-polymerase of mouse myeloma: partial purification and comparison of three molecular forms of the enzyme. Biochemistry. 1976 Nov 30;15(24):5305–5314. doi: 10.1021/bi00669a017. [DOI] [PubMed] [Google Scholar]
  9. Poonian M. S., Schlabach A. J., Weissbach A. Covalent attachment of nucleic acids to agarose for affinity chromatography. Biochemistry. 1971 Feb 2;10(3):424–427. doi: 10.1021/bi00779a011. [DOI] [PubMed] [Google Scholar]
  10. Potuzak H., Wintersberger U. DNA covalently linked to carboxymethyl-cellulose and its application in affinity chromatography. FEBS Lett. 1976 Mar 15;63(1):167–170. doi: 10.1016/0014-5793(76)80218-9. [DOI] [PubMed] [Google Scholar]
  11. Purifoy D. J., Powell K. L. DNA-binding proteins induced by herpes simplex virus type 2 in HEp-2 cells. J Virol. 1976 Aug;19(2):717–731. doi: 10.1128/jvi.19.2.717-731.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Rickwood D. An improved method for the insolubilization of DNA for affinity chromatography. Biochim Biophys Acta. 1972 Apr 26;269(1):47–50. doi: 10.1016/0005-2787(72)90072-x. [DOI] [PubMed] [Google Scholar]
  13. Rosen J. M., Barker S. W. Quantitation of casein messenger ribonucleic acid sequences using a specific complementary DNA hybridization probe. Biochemistry. 1976 Nov 30;15(24):5272–5280. doi: 10.1021/bi00669a012. [DOI] [PubMed] [Google Scholar]
  14. Rouvière-Yaniv J., Gros F. Characterization of a novel, low-molecular-weight DNA-binding protein from Escherichia coli. Proc Natl Acad Sci U S A. 1975 Sep;72(9):3428–3432. doi: 10.1073/pnas.72.9.3428. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Shih T. Y., Martin M. A. Chemical linkage of nucleic acids to neutral and phosphorylated cellulose powders and isolation of specific sequences by affinity chromatography. Biochemistry. 1974 Jul 30;13(16):3411–3418. doi: 10.1021/bi00713a036. [DOI] [PubMed] [Google Scholar]
  16. Stein G. H. Differences in DNA-binding proteins isolate from normal and transformed human cells. Exp Cell Res. 1976 Apr;99(1):115–125. doi: 10.1016/0014-4827(76)90686-8. [DOI] [PubMed] [Google Scholar]
  17. Weissbach A., Poonian M. Nucleic acids attached to solid matrices. Methods Enzymol. 1974;34:463–475. doi: 10.1016/s0076-6879(74)34057-8. [DOI] [PubMed] [Google Scholar]

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