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. 1978 Feb;5(2):583–590. doi: 10.1093/nar/5.2.583

A rapid method for the isolation of circular DNA using an aqueous two-phase partition system.

R Ohlsson, C C Hentschel, J G Williams
PMCID: PMC342003  PMID: 345246

Abstract

A method of isolating circular plasmid DNA from cleared lysates of E. coli is described. Purification is achieved by virtue of the rapid re-annealing kinetics or supercoiled DNA. After a brief denaturation step, double stranded plasmid DNA is separated from denatured chromosomal DNA and RNA in a two-phase partition system using dextran and polyethylene glycol. The method is much more rapid than the conventional dye-centrifugation technique and plasmid DNA of comparable purity and yield is obtained.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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