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. 1978 Jul;5(7):2441–2454. doi: 10.1093/nar/5.7.2441

RNA methylation in vaccinia virus-infected chick embryo fibroblasts treated with homologous interferon.

H Kroath, H J Gross, C Jungwirth, G Bodo
PMCID: PMC342175  PMID: 673858

Abstract

Interferon-pretreatment of vaccinia-infected chick embryo fibroblasts resulted in a greater than 50% decrease in ribose methylation of the penultimate "cap" nucleotide in virus-specific mRNA. However, in contrast to results obtained with cell-free systems, in intact infected cells there was (a) no detectable reduction in methylation of the 5'-ultimate m7G of viral mRNA; (b) a virus specificity of the interferon-induced change in mRNA "CAP"-methylation seems unlikely and (c) analysis of the ribosomal and transfer RNA fractions isolated from interferon-treated and control cells revealed identical patterns of methylated nucleotides. Thus, the interferon-induced change in methylation is specific for mRNA "CAPS".

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Selected References

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