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. 1978 Jul;5(7):2513–2524. doi: 10.1093/nar/5.7.2513

Relation of cell type and cell density in tissue culture to the isoaccepting spectra of the nucleoside Q containing tRNAs: tRNATyr, tRNAHis, tRNAAsn and tRNAAsp.

J R Katze
PMCID: PMC342181  PMID: 209410

Abstract

An examination, using reversed-phase chromatography and cyanogen bromide treatment, of tRNATyr, tRNAHis, tRNAAsn, and tRNAAsp from SV40-transformed mouse fibroblasts grown to different cell densities, untransformed cells grown to confluence, and mouse liver indicates that: (1) The tissue cultured mouse fibroblasts examined here are hypomodified with respect to nucleoside Q, while liver tRNA is almost completely modified with respect to Q. (2) Cell density and/or proliferative state do not present as major variables in controlling the expression of Q in the present system. (3) SV40 virus transformation is not a major variable controlling the expression of Q in the present system. The present results support previous use of cyanogen bromide effected shifts in chromatographic elution as an assay for nucleoside Q.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aaronson S. A., Todaro G. J. Development of 3T3-like lines from Balb-c mouse embryo cultures: transformation susceptibility to SV40. J Cell Physiol. 1968 Oct;72(2):141–148. doi: 10.1002/jcp.1040720208. [DOI] [PubMed] [Google Scholar]
  2. Brambilla R., Rogg H., Staehelin M. Unexpected occurrence of an aminoacylated nucleoside in mammalian tRNATyr. Nature. 1976 Sep 9;263(5573):167–169. doi: 10.1038/263167a0. [DOI] [PubMed] [Google Scholar]
  3. Briscoe W. T., Griffin A. C., McBride C., Bowen J. M. The distribution and properties of aspartyl transfer RNA in human and animal tumors. Cancer Res. 1975 Sep;35(9):2586–2593. [PubMed] [Google Scholar]
  4. Briscoe W. T., Syrewicz J. J., Marshall M. V., Griffin A. C. Regulation of an aspartyl-tRNA species in BHK cells in culture and in solid tumor form. Biochim Biophys Acta. 1975 Apr 2;383(4):441–445. doi: 10.1016/0005-2787(75)90314-7. [DOI] [PubMed] [Google Scholar]
  5. Ceccarini C., Eagle H. pH as a determinant of cellular growth and contact inhibition. Proc Natl Acad Sci U S A. 1971 Jan;68(1):229–233. doi: 10.1073/pnas.68.1.229. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dubrul E. F., Farkas W. R. Partial purification and properties of the reticulocyte guanylating enzyme. Biochim Biophys Acta. 1976 Sep 6;442(3):379–390. doi: 10.1016/0005-2787(76)90312-9. [DOI] [PubMed] [Google Scholar]
  7. Farkas W. R., Chernoff D. Identification of the minor guanylated tRNA of rabbit reticulocytes. Nucleic Acids Res. 1976 Oct;3(10):2521–2528. doi: 10.1093/nar/3.10.2521. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Friedman S., Li H. J., Nakanishi K., Van Lear G. 3-(3-amino-3-carboxy-n-propyl)uridine. The structure of the nucleoside in Escherichia coli transfer ribonucleic acid that reacts with phenoxyacetoxysuccinimide. Biochemistry. 1974 Jul 2;13(14):2932–2937. doi: 10.1021/bi00711a024. [DOI] [PubMed] [Google Scholar]
  9. Gallagher R. E., Ting R. C., Gallo R. C. A common change of aspartyl-tRNA in polyoma- and SV40 -transformed cells. Biochim Biophys Acta. 1972 Jul 31;272(4):568–582. doi: 10.1016/0005-2787(72)90512-6. [DOI] [PubMed] [Google Scholar]
  10. Kasai H., Nakanishi K., Macfarlane R. D., Torgerson D. F., Ohashi Z., McCloskey J. A., Gross H. J., Nishimura S. Letter: The structure of Q* nucleoside isolated from rabbit liver transfer ribonucleic acid. J Am Chem Soc. 1976 Aug 4;98(16):5044–5046. doi: 10.1021/ja00432a071. [DOI] [PubMed] [Google Scholar]
  11. Katze J. R. Alterations in SVT2 cell transfer RNAs in response to cell density and serum type. Biochim Biophys Acta. 1975 Mar 10;383(2):131–139. doi: 10.1016/0005-2787(75)90254-3. [DOI] [PubMed] [Google Scholar]
  12. Katze J. R., Mason K. H. Comparison of the acceptance activity of the ribosome-bound and the total cellular transfer ribonucleic acids from SV40-transformed mouse fibroblasts. Biochim Biophys Acta. 1973 Dec 21;331(3):369–381. doi: 10.1016/0005-2787(73)90023-3. [DOI] [PubMed] [Google Scholar]
  13. Katze J. R. Relation of cell type and cell density to the degree of post-transcriptional modification of tRNALys and tRNAPhe. Biochim Biophys Acta. 1975 Nov 4;407(4):392–398. doi: 10.1016/0005-2787(75)90291-9. [DOI] [PubMed] [Google Scholar]
  14. Okada N., Shindo-Okada N., Nishimura S. Isolation of mammalian tRNAAsp and tRNATyr by lectin-Sepharose affinity column chromatography. Nucleic Acids Res. 1977 Feb;4(2):415–423. doi: 10.1093/nar/4.2.415. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Porter K. R., Todaro G. J., Fonte V. A scanning electron microscope study of surface features of viral and spontaneous transformants of mouse Balb-3T3 cells. J Cell Biol. 1973 Dec;59(3):633–642. doi: 10.1083/jcb.59.3.633. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Roe B. A., Stankiewicz A. F., Chen C. Y. Chromatographic behavior of several mammalian tRNAs on acylated dihydroxyl-borate cellulose and Aminex A-28. Nucleic Acids Res. 1977 Jul;4(7):2191–2204. doi: 10.1093/nar/4.7.2191. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. White B. N. Chromatographic changes in specific tRNAs after reaction with cyanogen bromide and sodium periodate. Biochim Biophys Acta. 1974 Jul 11;353(3):283–291. doi: 10.1016/0005-2787(74)90021-5. [DOI] [PubMed] [Google Scholar]

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