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. 1978 Aug;5(8):2705–2720. doi: 10.1093/nar/5.8.2705

Evidence for RNA-RNA cross-link formation in Escherichia coli ribosomes.

C Zwieb, A Ross, J Rinke, M Meinke, R Brimacombe
PMCID: PMC342201  PMID: 358146

Abstract

Evidence is presented in three separate cases for the formation of RNA-RNA cross-links in intact E. coli ribosomes and ribosomal subunits. The first case is a cross-link between the 18S and 13S regions of the 23S RNA, induced by ultraviolet irradiation. The second is a cross-link at the subunit interface, generated by the bifunctional reagent bis-(2-chloroethyl)-amine. The third example is a cross-link between sections O'-D and P-A of the 16S RNA, induced as in the first case by ultraviolet irradiation. The RNA-RNA cross-links can be identified as such, despite the complications introduced by concomitant RNA-protein cross-linking reactions. The experiments represent a first attempt to introduce RNA-RNA cross-linking into studies of the topographical organization of the RNA within the ribosome.

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Selected References

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