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. 1978 Sep;5(9):3183–3194. doi: 10.1093/nar/5.9.3183

Demonstration of high molecular weight poly (adenosine diphosphate ribose).

M Tanaka, K Hayashi, H Sakura, M Miwa, T Matsushima, T Sugimura
PMCID: PMC342240  PMID: 704350

Abstract

An electrophoretic system was established that resolves poly(adenosine diphosphate ribose), enzymatically synthesized polymer from NAD+, by size difference of one residue on polyacrylamide gel. The existence of a polymer of at least 65 residues was demonstrated by band counting in this system. The polymer showed a heterogeneous size distribution on the electrophoregram, and the molecular weight of the largest polymer was deduced to be more than 4.5 X 10(5) daltons. The discrepancy between the size, estimated by electrophoresis, and the chain length, determined by the ratio of total radioactivity to that derived from the terminus, suggests that the polymer has a branched structure.

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Selected References

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