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. 1979 Oct 25;7(4):959–970. doi: 10.1093/nar/7.4.959

The nucleotide sequence of human tRNAGly (anticodon GCC).

R C Gupta, B A Roe, K Randerath
PMCID: PMC342274  PMID: 116197

Abstract

The sequence of tRNAGCCGly from human placenta was determined by recently developed postlabeling techniques. The tRNA was digested completely with RNases T1 and A in the presence of alkaline phosphatase, the oligonucleotides were 3'-terminally (3H)-labeled, mapped on PEI-cellulose thin layers, isolated, and sequenced by methods based on base-specific cleavages. Overlaps were obtained by readout sequencing techniques on polyacrylamide gels and PEI-cellulose thin layers. The thin-layer readout technique was used also to locate and identify modified nucleotides. The primary structure was found to exhibit a large degree of homology (94.6%) with silkworm tRNAGCCGly but only 67.6% homology with human tRNACCCGly.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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