Abstract
Spermine-DNA complexes have been examined by small-angle and wide-angle X-ray scattering as well as by circular dichroism studies. Condensed complexes are building up below a critical ionic strength. We have found that at one and the same ionic strength condensed complexes having two different supramolecular structures (Type I and Type II) can coexist. The structure of the condensates depends on the method of condensate formation. Phase transitions between these structures can be induced by thermal treatment. We conclude from these facts that with polyamine-DNA condensates metastable structures are of importance.
Full text
PDFSelected References
These references are in PubMed. This may not be the complete list of references from this article.
- Chattoraj D. K., Gosule L. C., Schellman A. DNA condensation with polyamines. II. Electron microscopic studies. J Mol Biol. 1978 May 25;121(3):327–337. doi: 10.1016/0022-2836(78)90367-4. [DOI] [PubMed] [Google Scholar]
- Damaschun H., Damaschun G., Becker M., Buder E., Misselwitz R., Zirwer D. Sekundärstruktur kondensierter DNS. Vergleichende Untersuchungen der diffusen Röntgen-Weitwinkelstreuung und des Zirkulardichroismus. Acta Biol Med Ger. 1978;37(4):569–576. [PubMed] [Google Scholar]
- Damaschun H., Damaschun G., Becker M., Buder E., Misselwitz R., Zirwer D. Study of DNA-spermine interactions by use of small-angle and wide-angle X-ray scattering and circular dichroism. Nucleic Acids Res. 1978 Oct;5(10):3801–3809. doi: 10.1093/nar/5.10.3801. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Eickbush T. H., Moudrianakis E. N. The compaction of DNA helices into either continuous supercoils or folded-fiber rods and toroids. Cell. 1978 Feb;13(2):295–306. doi: 10.1016/0092-8674(78)90198-8. [DOI] [PubMed] [Google Scholar]
- Giannoni G., Padden F. J., Jr, Keith H. D. Crystallization of DNA from dilute solution. Proc Natl Acad Sci U S A. 1969 Mar;62(3):964–971. doi: 10.1073/pnas.62.3.964. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gosule L. C., Schellman J. A. Compact form of DNA induced by spermidine. Nature. 1976 Jan 29;259(5541):333–335. doi: 10.1038/259333a0. [DOI] [PubMed] [Google Scholar]
- Gosule L. C., Schellman J. A. DNA condensation with polyamines I. Spectroscopic studies. J Mol Biol. 1978 May 25;121(3):311–326. doi: 10.1016/0022-2836(78)90366-2. [DOI] [PubMed] [Google Scholar]
- Hochkeppel H. K., Craven G. R. Significant changes in 16 S RNA conformation accompanying assembly of the 30 S ribosome in vitro. J Mol Biol. 1977 Jul 15;113(4):623–634. doi: 10.1016/0022-2836(77)90226-1. [DOI] [PubMed] [Google Scholar]
- Kawashima S., Ando T. Interaction of basic oligo-L-amino acids with deoxyribonucleic acids. Oligo-L-arginines of various chain lengths and herring sperm DNA. J Biochem. 1978 Aug;84(2):343–350. doi: 10.1093/oxfordjournals.jbchem.a132134. [DOI] [PubMed] [Google Scholar]
- Lerman L. S. A transition to a compact form of DNA in polymer solutions. Proc Natl Acad Sci U S A. 1971 Aug;68(8):1886–1890. doi: 10.1073/pnas.68.8.1886. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Osland A., Kleppe K. Polyamine induced aggregation of DNA. Nucleic Acids Res. 1977 Mar;4(3):685–695. doi: 10.1093/nar/4.3.685. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rubin R. L. Spermidine-Deoxyribonucleic acid interaction in vitro and in Escherichia coli. J Bacteriol. 1977 Feb;129(2):916–925. doi: 10.1128/jb.129.2.916-925.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zeppezauer M., Eklund H., Zeppezauer E. S. Micro diffusion cells for the growth of single protein crystals by means of equilibrium dialysis. Arch Biochem Biophys. 1968 Aug;126(2):564–573. doi: 10.1016/0003-9861(68)90443-8. [DOI] [PubMed] [Google Scholar]