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. 1979 Dec 20;7(8):2137–2146. doi: 10.1093/nar/7.8.2137

Southern bean mosaic viral RNA has a 5'-linked protein but lacks 3' terminal poly(A).

A Ghosh, R Dasgupta, T Salerno-Rife, T Rutgers, P Kaesberg
PMCID: PMC342375  PMID: 523316

Abstract

Nuclease digestion of SBMV RNA releases a protein of molecular weight approximately 12,000 in addition to the four mononucleotides. The lack of reactivity of SBMV RNA to polynucleotide kinase and the absence of a capping group suggest that the protein is covalently attached to the 5' end of the RNA. RNA sequencing shows that the 3' terminus of SBMV RNA is not polyadenylated.

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Selected References

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  1. Black D. N., Burroughs J. N., Harris T. J., Brown F. The structure and replication of calicivirus RNA. Nature. 1978 Aug 10;274(5671):614–615. doi: 10.1038/274614a0. [DOI] [PubMed] [Google Scholar]
  2. Burroughs J. N., Brown F. Presence of a covalently linked protein on calicivirus RNA. J Gen Virol. 1978 Nov;41(2):443–446. doi: 10.1099/0022-1317-41-2-443. [DOI] [PubMed] [Google Scholar]
  3. Daubert S. D., Bruening G., Najarian R. C. Protein bound to the genome RNAs of cowpea mosaic virus. Eur J Biochem. 1978 Dec 1;92(1):45–51. doi: 10.1111/j.1432-1033.1978.tb12721.x. [DOI] [PubMed] [Google Scholar]
  4. Flanegan J. B., Petterson R. F., Ambros V., Hewlett N. J., Baltimore D. Covalent linkage of a protein to a defined nucleotide sequence at the 5'-terminus of virion and replicative intermediate RNAs of poliovirus. Proc Natl Acad Sci U S A. 1977 Mar;74(3):961–965. doi: 10.1073/pnas.74.3.961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hruby D. E., Roberts W. K. Encephalomyocarditis virus RNA. III. Presence of a genome-associated protein. J Virol. 1978 Jan;25(1):413–415. doi: 10.1128/jvi.25.1.413-415.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hruby D. E., Roberts W. K. Encephalomyocarditis virus RNA: variations in polyadenylic acid content and biological activity. J Virol. 1976 Aug;19(2):325–330. doi: 10.1128/jvi.19.2.325-330.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hull R. The banding behaviour of the viruses of southern bean mosaic virus group in gradients of caesium sulphate. Virology. 1977 Jun 1;79(1):50–57. doi: 10.1016/0042-6822(77)90333-6. [DOI] [PubMed] [Google Scholar]
  8. Laskey R. A., Mills A. D. Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography. Eur J Biochem. 1975 Aug 15;56(2):335–341. doi: 10.1111/j.1432-1033.1975.tb02238.x. [DOI] [PubMed] [Google Scholar]
  9. Lee Y. F., Nomoto A., Detjen B. M., Wimmer E. A protein covalently linked to poliovirus genome RNA. Proc Natl Acad Sci U S A. 1977 Jan;74(1):59–63. doi: 10.1073/pnas.74.1.59. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Means G. E., Feeney R. E. Reductive alkylation of amino groups in proteins. Biochemistry. 1968 Jun;7(6):2192–2201. doi: 10.1021/bi00846a023. [DOI] [PubMed] [Google Scholar]
  11. Nishimura S. Minor components in transfer RNA: their characterization, location, and function. Prog Nucleic Acid Res Mol Biol. 1972;12:49–85. [PubMed] [Google Scholar]
  12. Nomoto A., Kitamura N., Golini F., Wimmer E. The 5'-terminal structures of poliovirion RNA and poliovirus mRNA differ only in the genome-linked protein VPg. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5345–5349. doi: 10.1073/pnas.74.12.5345. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Peacock A. C., Dingman C. W. Molecular weight estimation and separation of ribonucleic acid by electrophoresis in agarose-acrylamide composite gels. Biochemistry. 1968 Feb;7(2):668–674. doi: 10.1021/bi00842a023. [DOI] [PubMed] [Google Scholar]
  14. Rayment I., Johnson J. E., Rossmann M. G. Metal-free southern bean mosaic virus crystals. J Biol Chem. 1979 Jun 25;254(12):5243–5245. [PubMed] [Google Scholar]
  15. Rice R. H., Means G. E. Radioactive labeling of proteins in vitro. J Biol Chem. 1971 Feb 10;246(3):831–832. [PubMed] [Google Scholar]
  16. Sangar D. V., Rowlands D. J., Harris T. J., Brown F. Protein covalently linked to foot-and-mouth disease virus RNA. Nature. 1977 Aug 18;268(5621):648–650. doi: 10.1038/268648a0. [DOI] [PubMed] [Google Scholar]
  17. Shatkin A. J. Capping of eucaryotic mRNAs. Cell. 1976 Dec;9(4 Pt 2):645–653. doi: 10.1016/0092-8674(76)90128-8. [DOI] [PubMed] [Google Scholar]
  18. Silberklang M., Prochiantz A., Haenni A. L., Rajbhandary U. L. Studies on the sequence of the 3'-terminal region of turnip-yellow-mosaic-virus RNA. Eur J Biochem. 1977 Feb;72(3):465–478. doi: 10.1111/j.1432-1033.1977.tb11270.x. [DOI] [PubMed] [Google Scholar]
  19. Stanley J., Rottier P., Davies J. W., Zabel P., Van Kammen A. A protein linked to the 5' termini of both RNA components of the cowpea mosaic virus genome. Nucleic Acids Res. 1978 Dec;5(12):4505–4522. doi: 10.1093/nar/5.12.4505. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Swank R. T., Munkres K. D. Molecular weight analysis of oligopeptides by electrophoresis in polyacrylamide gel with sodium dodecyl sulfate. Anal Biochem. 1971 Feb;39(2):462–477. doi: 10.1016/0003-2697(71)90436-2. [DOI] [PubMed] [Google Scholar]
  21. Yogo Y., Wimmer E. Polyadenylic acid at the 3'-terminus of poliovirus RNA. Proc Natl Acad Sci U S A. 1972 Jul;69(7):1877–1882. doi: 10.1073/pnas.69.7.1877. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Zimmern D. The 5' end group of tobacco mosaic virus RNA is m7G5' ppp5' Gp. Nucleic Acids Res. 1975 Jul;2(7):1189–1201. doi: 10.1093/nar/2.7.1189. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. el-Manna M. M., Bruening G. Polyadenylate sequences in the ribonucleic acids of cowpea mosaic virus. Virology. 1973 Nov;56(1):198–206. doi: 10.1016/0042-6822(73)90299-7. [DOI] [PubMed] [Google Scholar]

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