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. 1977 Jan;4(1):223–228. doi: 10.1093/nar/4.1.223

DNA swivel enzyme activity in a nuclear membrane fraction.

S Yoshida, G Ungers, B H Rosenberg
PMCID: PMC342421  PMID: 871320

Abstract

DNA swivel (nicking-rejoining) enzyme activity has been studied in various cell fractions of a human lymphoid cell line. Swivel activity is found only in chromatin and in a nuclear membrane fraction containing DNA and possessing endogenous DNA synthesizing activity. Twenty percent of the total swivel activity and less than one percent of the total DNA are in the membrane fraction. The swivel enzyme is more tightly bound to the membrane fraction than to the chromatin fraction. These observations suggest that the swivel enzyme may be a replication factor, specifically bound to replicating DNA in the membrane fraction.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baase W. A., Wang J. C. An omega protein from Drosophila melanogaster. Biochemistry. 1974 Oct 8;13(21):4299–4303. doi: 10.1021/bi00718a009. [DOI] [PubMed] [Google Scholar]
  2. Cavalieri L. F., Carroll E. New DNA polymerase activity associated with nuclear membranes from a human lymphoid cell line. Biochem Biophys Res Commun. 1975 Dec 15;67(4):1360–1369. doi: 10.1016/0006-291x(75)90177-1. [DOI] [PubMed] [Google Scholar]
  3. Champoux J. J., Dulbecco R. An activity from mammalian cells that untwists superhelical DNA--a possible swivel for DNA replication (polyoma-ethidium bromide-mouse-embryo cells-dye binding assay). Proc Natl Acad Sci U S A. 1972 Jan;69(1):143–146. doi: 10.1073/pnas.69.1.143. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Keller W. Characterization of purified DNA-relaxing enzyme from human tissue culture cells. Proc Natl Acad Sci U S A. 1975 Jul;72(7):2550–2554. doi: 10.1073/pnas.72.7.2550. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Keller W. Determination of the number of superhelical turns in simian virus 40 DNA by gel electrophoresis. Proc Natl Acad Sci U S A. 1975 Dec;72(12):4876–4880. doi: 10.1073/pnas.72.12.4876. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Keller W., Wendel I. Stepwise relaxation of supercoiled SV40 DNA. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 1):199–208. doi: 10.1101/sqb.1974.039.01.026. [DOI] [PubMed] [Google Scholar]
  7. Vosberg H. P., Grossman L. I., Vinograd J. Isolation and partial characterisation of the relaxation protein from nuclei of cultured mouse and human cells. Eur J Biochem. 1975 Jun 16;55(1):79–93. doi: 10.1111/j.1432-1033.1975.tb02140.x. [DOI] [PubMed] [Google Scholar]
  8. Vosberg H. P., Vinograd J. Purification and demonstration of the enzymatic character of the nicking-closing protein from mouse L cells. Biochem Biophys Res Commun. 1976 Jan 26;68(2):456–464. doi: 10.1016/0006-291x(76)91167-0. [DOI] [PubMed] [Google Scholar]
  9. Wang J. C. Interaction between DNA and an Escherichia coli protein omega. J Mol Biol. 1971 Feb 14;55(3):523–533. doi: 10.1016/0022-2836(71)90334-2. [DOI] [PubMed] [Google Scholar]

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