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. 1977 Feb;4(2):327–338. doi: 10.1093/nar/4.2.327

Fluoresceinylthiocarbamyl-tRNATyr: a useful derivative of tRNATyr (E.coli) for physicochemical studies.

A Pingoud, R Kownatzki, G Maass
PMCID: PMC342435  PMID: 14327

Abstract

Fluoresceinylthiocarbamyl-tRNATyr (FTC-tRNATyr) is prepared from tRNATyr and fluoresceinisothiogyanate (FITC) under mildly alkaline conditions. Labelling occurs specificly at the base Q of tRNATyr. The modified tRNA is fully active in the aminoacylation assay; when aminoacylated it is recognized by the elongation factor Tu (EF-Tu). Codon-anticodon interaction, however, is severely affected by the modification.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beres L., Lucas-Lenard J. Studies on the fluorescence of the Y base of yeast phenylalanine transfer ribonucleic acid. Effect of pH, aminoacylation, and interaction with elongation factor Tu. Biochemistry. 1973 Sep 25;12(20):3998–4002. doi: 10.1021/bi00744a033. [DOI] [PubMed] [Google Scholar]
  2. Eisinger J., Gross N. The anticodon-anticodon complex. J Mol Biol. 1974 Sep 5;88(1):165–174. doi: 10.1016/0022-2836(74)90302-7. [DOI] [PubMed] [Google Scholar]
  3. Harada F., Kimura F., Nishimura S. Nucleotide sequence of valine tRNA 1 from Escherichia coli B. Biochim Biophys Acta. 1969 Dec 16;195(2):590–592. doi: 10.1016/0005-2787(69)90671-6. [DOI] [PubMed] [Google Scholar]
  4. Kasai H., Kuchino Y., Nihei K., Nishimura S. Distribution of the modified nucleoside Q and its derivatives in animal and plant transfer RNA's. Nucleic Acids Res. 1975 Oct;2(10):1931–1939. doi: 10.1093/nar/2.10.1931. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kasai H., Oashi Z., Harada F., Nishimura S., Oppenheimer N. J., Crain P. F., Liehr J. G., von Minden D. L., McCloskey J. A. Structure of the modified nucleoside Q isolated from Escherichia coli transfer ribonucleic acid. 7-(4,5-cis-Dihydroxy-1-cyclopenten-3-ylaminomethyl)-7-deazaguanosine. Biochemistry. 1975 Sep 23;14(19):4198–4208. doi: 10.1021/bi00690a008. [DOI] [PubMed] [Google Scholar]
  6. Landel A. M. Stability studies on fluorescein isothiocyanate--bovine serum albumin conjugate. Anal Biochem. 1976 Jun;73(2):280–289. doi: 10.1016/0003-2697(76)90173-1. [DOI] [PubMed] [Google Scholar]
  7. Miller D. L., Weissbach H. Studies on the purification and properties of factor Tu from E. coli. Arch Biochem Biophys. 1970 Nov;141(1):26–37. doi: 10.1016/0003-9861(70)90102-5. [DOI] [PubMed] [Google Scholar]
  8. Philippsen P., Zachau G. Partial degradation of transfer RNAs and transfer RNA fragments by spleen phosphodiesterase as studied by disc electrophoretic methods. Biochim Biophys Acta. 1972 Sep 14;277(3):523–538. doi: 10.1016/0005-2787(72)90095-0. [DOI] [PubMed] [Google Scholar]
  9. Randerath E., Yu C. T., Randerath K. Base analysis of ribopolynucleotides by chemical tritium labeling: a methodological study with model nucleosides and purified tRNA species. Anal Biochem. 1972 Jul;48(1):172–198. doi: 10.1016/0003-2697(72)90181-9. [DOI] [PubMed] [Google Scholar]
  10. Riesner D., Pingoud A., Boehme D., Peters F., Maass G. Distinct steps in the specific binding of tRNA to aminoacyl-tRNA synthetase. Temperature-jump studies on the serine-specific system from yeast and the tyrosine-specific system from Escherichia coli. Eur J Biochem. 1976 Sep;68(1):71–80. doi: 10.1111/j.1432-1033.1976.tb10765.x. [DOI] [PubMed] [Google Scholar]

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