Skip to main content
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1977 Mar;4(3):503–511. doi: 10.1093/nar/4.3.503

Proflavine sensitivity of RNA processing in isolated nuclei.

A Yannarell, M Niemann, D E Schumm, T E Webb
PMCID: PMC342457  PMID: 866181

Abstract

The intercalating agent proflavine inhibits the processing and subsequent release of preformed messenger RNA and ribosomal RNA from isolated liver nuclei to surrogate cytoplasm. The direct effect of proflavine on these processes, as monitored in a reconstituted cell-free system, supports the theory that base-paired segments (i.e. hairpin loops) in the precursor RNA's are involved as recognition sites in nuclear RNA processing.

Full text

PDF
504

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brawerman G. Eukaryotic messenger RNA. Annu Rev Biochem. 1974;43(0):621–642. doi: 10.1146/annurev.bi.43.070174.003201. [DOI] [PubMed] [Google Scholar]
  2. Brawerman G. The isolation of messenger RNA from mammalian cells. Methods Enzymol. 1974;30:605–612. doi: 10.1016/0076-6879(74)30058-4. [DOI] [PubMed] [Google Scholar]
  3. Brinker J. M., Madore H. P., Bello L. J. Stabilization of heterogeneous nuclear RNA by intercalating drugs. Biochem Biophys Res Commun. 1973 Jun 8;52(3):928–934. doi: 10.1016/0006-291x(73)91026-7. [DOI] [PubMed] [Google Scholar]
  4. Darnell J. E., Philipson L., Wall R., Adesnik M. Polyadenylic acid sequences: role in conversion of nuclear RNA into messenger RNA. Science. 1971 Oct 29;174(4008):507–510. doi: 10.1126/science.174.4008.507. [DOI] [PubMed] [Google Scholar]
  5. Jelinek W., Darnell J. E. Double-stranded regions in heterogeneous nuclear RNA from Hela cells. Proc Natl Acad Sci U S A. 1972 Sep;69(9):2537–2541. doi: 10.1073/pnas.69.9.2537. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Naora H., Whitelam J. M. Presence of sequences hybridisable to dsRNA in cytoplasmic mRNA molecules. Nature. 1975 Aug 28;256(5520):756–759. doi: 10.1038/256756a0. [DOI] [PubMed] [Google Scholar]
  7. Penman S., Vesco C., Penman M. Localization and kinetics of formation of nuclear heterodisperse RNA, cytoplasmic heterodisperse RNA and polyribosome-associated messenger RNA in HeLa cells. J Mol Biol. 1968 May 28;34(1):49–60. doi: 10.1016/0022-2836(68)90234-9. [DOI] [PubMed] [Google Scholar]
  8. Racevskis J., Webb T. E. Processing and release of ribosomal RNA from isolated nuclei: analysis of the ATP-dependence and cytosol-dependence. Eur J Biochem. 1974 Nov 1;49(1):93–100. doi: 10.1111/j.1432-1033.1974.tb03814.x. [DOI] [PubMed] [Google Scholar]
  9. Rizzo A. J., Webb T. E. Regulation of ribosome formation in regenerating rat liver. Eur J Biochem. 1972 May;27(1):136–144. doi: 10.1111/j.1432-1033.1972.tb01819.x. [DOI] [PubMed] [Google Scholar]
  10. Rottman F., Shatkin A. J., Perry R. P. Sequences containing methylated nucleotides at the 5' termini of messenger RNAs: possible implications for processing. Cell. 1974 Nov;3(3):197–199. doi: 10.1016/0092-8674(74)90131-7. [DOI] [PubMed] [Google Scholar]
  11. Schumm D. E., McNamara D. J., Webb T. E. Cytoplasmic proteins regulating messenger RNA release from nuclei. Nat New Biol. 1973 Oct 17;245(146):201–203. doi: 10.1038/newbio245201a0. [DOI] [PubMed] [Google Scholar]
  12. Schumm D. E., Webb T. E. Modified messenger ribonucleic acid release from isolated hepatic nuclei after inhibition of polyadenylate formation. Biochem J. 1974 Apr;139(1):191–196. doi: 10.1042/bj1390191. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Schumm D. E., Webb T. E. The in vivo equivalence of a cell-free system for RNA processing and transport. Biochem Biophys Res Commun. 1974 May 20;58(2):354–360. doi: 10.1016/0006-291x(74)90372-6. [DOI] [PubMed] [Google Scholar]
  14. Schumm D. E., Webb T. E. Transport of informosomes from isolated nuclei of regenerating rat liver. Biochem Biophys Res Commun. 1972 Sep 5;48(5):1259–1265. doi: 10.1016/0006-291x(72)90847-9. [DOI] [PubMed] [Google Scholar]
  15. Snyder A. L., Kann H. E., Jr, Kohn K. W. Inhibition of the processing of ribosomal precursor RNA by intercalating agents. J Mol Biol. 1971 Jun 14;58(2):555–565. doi: 10.1016/0022-2836(71)90371-8. [DOI] [PubMed] [Google Scholar]
  16. Weinberg R. A., Penman S. Processing of 45 s nucleolar RNA. J Mol Biol. 1970 Jan 28;47(2):169–178. doi: 10.1016/0022-2836(70)90337-2. [DOI] [PubMed] [Google Scholar]
  17. Zimmerman E. F., Holler B. W. Methylation of 45 s ribosomal RNA precursor in HeLa cells. J Mol Biol. 1967 Jan 28;23(2):149–161. doi: 10.1016/s0022-2836(67)80023-8. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES