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. 1977 Apr;4(4):801–811. doi: 10.1093/nar/4.4.801

Histone mRNA in Xenopus laevis ovaries: identification of the H4 messenger.

O H Destrée, A L Haenni, M L Birnstiel
PMCID: PMC342485  PMID: 866192

Abstract

RNA extracted from ovaries of adult Xenopus laevis and introduced into a wheat germ cell-free system, directs the synthesis of polypeptides co-electrophoresing with all five histones. By frationation of total ovary RNA through Sephadex G-200 and polyacrylamide gel, RNA was isolated that is translated into histones 4 (H4) only. In addition, this RNA labelled in vitro with [I125] hybridizes to a restriction fragment of cloned sea urchin DNA known to contain the H4 gene.

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Selected References

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