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. 1977 Apr;4(4):917–928. doi: 10.1093/nar/4.4.917

Effect of homopolyribonucleotides on messenger ribonucleoprotein particles.

B Sundquist, T Persson
PMCID: PMC342494  PMID: 866198

Abstract

A discrete set of polypeptides copurify with and appear to be specifically attached to mRNA from polysomes of eukaryotic cells. This report describes the effect of homopolyribonucleotides on mRNA-protein complexes separated from ribosome subunits by oligo(dT)-cellulose chromatography. It is shown that poly (U) and poly (A) can release mRNA-protein complexes adsorbed to oligo(dT)-cellulose, whereas poly (C) and poly(I) are much less effective in this process. Analysis of polyribonucleotide released material showed that poly(U) effectively dissociated the mRNA-protein complexes while poly(A) caused no or only partial derangement of these particles. The specificities seen in the polyribonucleotide effects in turn suggest a high degree of specificity in the interaction between the proteins and mRNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baltimore D., Huang A. S. Isopycnic separation of subcellular components from poliovirus-infected and normal HeLa cells. Science. 1968 Nov 1;162(3853):572–574. doi: 10.1126/science.162.3853.572. [DOI] [PubMed] [Google Scholar]
  2. Bishop J. O., Rosbash M. Polynucleotide sequences in eukaryotic DNA and RNA that form ribonuclease-resistant complexes with polyuridylic acid. J Mol Biol. 1974 May 5;85(1):75–86. doi: 10.1016/0022-2836(74)90130-2. [DOI] [PubMed] [Google Scholar]
  3. Blobel G. A protein of molecular weight 78,000 bound to the polyadenylate region of eukaryotic messenger RNAs. Proc Natl Acad Sci U S A. 1973 Mar;70(3):924–928. doi: 10.1073/pnas.70.3.924. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Both G. W., Furuichi Y., Muthukrishnan S., Shatkin A. J. Ribosome binding to reovirus mRNA in protein synthesis requires 5' terminal 7-methylguanosine. Cell. 1975 Oct;6(2):185–195. doi: 10.1016/0092-8674(75)90009-4. [DOI] [PubMed] [Google Scholar]
  5. Dasgupta R., Shih D. S., Saris C., Kaesberg P. Nucleotide sequence of a viral RNA fragment that binds to eukaryotic ribosomes. Nature. 1975 Aug 21;256(5519):624–628. doi: 10.1038/256624a0. [DOI] [PubMed] [Google Scholar]
  6. Ernst V., Arnstein H. R. Synthesis of alpha- and beta-globin directed by messenger ribonucleoprotein from rabbit reticulocytes. Biochim Biophys Acta. 1975 Jan 20;378(2):251–259. doi: 10.1016/0005-2787(75)90113-6. [DOI] [PubMed] [Google Scholar]
  7. Everitt E., Philipson L. Structural proteins of adenoviruses. XI. Purification of three low molecular weight virion proteins of adenovirus type 2 and their synthesis during productive infection. Virology. 1974 Nov;62(1):253–269. doi: 10.1016/0042-6822(74)90320-1. [DOI] [PubMed] [Google Scholar]
  8. Favre A., Morel C., Scherrer K. The secondary structure and poly(A) content of globin messenger RNA as a pure RNA and in polyribosome-derived ribonucleoprotein complexes. Eur J Biochem. 1975 Sep 1;57(1):147–157. doi: 10.1111/j.1432-1033.1975.tb02285.x. [DOI] [PubMed] [Google Scholar]
  9. Greenberg J. R., Perry R. P. Relative occurrence of polyadenylic acid sequences in messenger and heterogeneous nuclear RNA of L cells as determined by poly (U)-hydroxylapatite chromatography. J Mol Biol. 1972 Dec 14;72(1):91–98. doi: 10.1016/0022-2836(72)90070-8. [DOI] [PubMed] [Google Scholar]
  10. Hendrick D., Schwarz W., Pitzel S., Tiedemann H. Messenger ribonucleoprotein-directed globin synthesis in an embryonic brain cell-free system. Biochim Biophys Acta. 1974 Mar 27;340(3):278–284. doi: 10.1016/0005-2787(74)90273-1. [DOI] [PubMed] [Google Scholar]
  11. Irwin D., Kumar A., Malt R. A. Messenger ribonucleoprotein complexes isolated with oligo(dT)-cellulose chromatography from kidney polysomes. Cell. 1975 Feb;4(2):157–165. doi: 10.1016/0092-8674(75)90122-1. [DOI] [PubMed] [Google Scholar]
  12. Kumar A., Lindberg U. Characterization of messenger ribonucleoprotein and messenger RNA from KB cells. Proc Natl Acad Sci U S A. 1972 Mar;69(3):681–685. doi: 10.1073/pnas.69.3.681. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kumar A., Pederson T. Comparison of proteins bound to heterogeneous nuclear RNA and messenger RNA in HeLa cells. J Mol Biol. 1975 Aug 15;96(3):353–365. doi: 10.1016/0022-2836(75)90165-5. [DOI] [PubMed] [Google Scholar]
  14. Kwan S. W., Brawerman G. A particle associated with the polyadenylate segment in mammalian messenger RNA. Proc Natl Acad Sci U S A. 1972 Nov;69(11):3247–3250. doi: 10.1073/pnas.69.11.3247. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Lindberg U., Persson T., Philipson L. Isolation and characterization of adenovirus messenger ribonucleic acid in productive infection. J Virol. 1972 Nov;10(5):909–919. doi: 10.1128/jvi.10.5.909-919.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Lindberg U., Sundquist B. Isolation of messenger ribonucleoproteins from mammalian cells. J Mol Biol. 1974 Jun 25;86(2):451–468. doi: 10.1016/0022-2836(74)90030-8. [DOI] [PubMed] [Google Scholar]
  17. Lingrel J. B., Lockard R. E., Jones R. F., Burr H. E., Holder J. W. Biologically active messenger-RNA for hemoglobin. Ser Haematol. 1971;4(3):37–69. [PubMed] [Google Scholar]
  18. Nudel U., Lebleu B., Zehavi-Willner T., Revel M. Mesenger ribonucleoprotein and initiation factors in rabbit-reticulocyte polyribosomes. Eur J Biochem. 1973 Mar 1;33(2):314–322. doi: 10.1111/j.1432-1033.1973.tb02685.x. [DOI] [PubMed] [Google Scholar]
  19. Rosbash M. Polyadenylic acid-containing RNA in Xenopus laevis oocytes. J Mol Biol. 1974 May 5;85(1):87–101. doi: 10.1016/0022-2836(74)90131-4. [DOI] [PubMed] [Google Scholar]
  20. Sampson J., Mathews M. B., Osborn M., Borghetti A. F. Hemoglobin messenger ribonucleic acid translation in cell-free systems from rat and mouse liver and Landschutz ascites cells. Biochemistry. 1972 Sep 12;11(19):3636–3640. doi: 10.1021/bi00769a022. [DOI] [PubMed] [Google Scholar]
  21. Sundquist B., Persson T., Lindberg U. Characterization of mRNA-protein complexes from mammalian cells. Nucleic Acids Res. 1977 Apr;4(4):899–915. doi: 10.1093/nar/4.4.899. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Van der Marel P., Tasseron-de Jong J. G., Bosch L. The proteins associated with mRNA from uninfected and adenovirus type 5-infected KB cells. FEBS Lett. 1975 Mar 1;51(1):330–334. doi: 10.1016/0014-5793(75)80919-7. [DOI] [PubMed] [Google Scholar]

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