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. 1977 Jun;4(6):1803–1813. doi: 10.1093/nar/4.6.1803

Relaxed circular SV40 DNA as cleavage intermediate of two restriction endonucleases.

G Ruben, P Spielman, C D Tu, E Jay, B Siegel, R Wu
PMCID: PMC342523  PMID: 197493

Abstract

We have determined the mode of cleavage of superhelical SV40 DNA (Form I) by restriction endonucleases EcoRI and HpaII at 37 degrees C. By analysis with agarose gel electrophoresis and direct examination with dark field electron microscopy, we found that a large amount of the single-nicked circular DNA (Form II) was produced before the linear SV40 DNA (Form III) appeared. Thus, both restriction enzymes cleave only one strand of the superhelical DNA first. The second cleavage on the complementary strand occurred after a lag period. The first order rate constant for the second cleavage by EcoRI endonuclease was determined and a kinetic reaction scheme for both enzymes is proposed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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