Skip to main content
PMC home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1977 Jul;4(7):2353–2368. doi: 10.1093/nar/4.7.2353

Nucleotide sequence of 3' untranslated portion of human alpha globin mRNA.

J T Wilson, J K deRiel, B G Forget, C A Marotta, S M Weissman
PMCID: PMC342571  PMID: 909779

Abstract

We have determined the nucleotide sequence of 75 nucleotides of the 3'-untranslated portion of normal human alpha globin mRNA which corresponds to the elongated amino acid sequence of the chain termination mutant Hb Constant Spring. This was accomplished by sequence analysis of cDNA fragments obtained by restriction endonuclease or T4 endonuclease IV cleavage of human globin cDNA synthesized from globin mRNA by use of viral reverse transcriptase. Analysis of cRNA synthesized from cDNA by use of RNA polymerase provided additional confirmatory sequence information. Possible polymorphism has been identified at one site of the sequence. Our sequence overlaps with, and extends the sequence of 43 nucleotides determined by Proudfood and coworkers for the very 3'-terminal portion of human alpha globin mRNA. The complete 3'-untranslated sequence of human alpha globin mRNA (112 nucleotides including termination codon) shows little homology to that of the human or rabbit beta globin mRNAs except for the presence of the hexanucleotide sequence AAUAAA which is found in most eukaryotic mRNAs near the 3'-terminal poly (A).

Full text

PDF
2357

Images in this article

2357Image
on p.2357
2357Image
on p.2357
2358Image
on p.2358
2358Image
on p.2358
2359Image
on p.2359
2359Image
on p.2359
2360Image
on p.2360

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Clegg J. B., Weatherall D. J., Milner P. F. Haemoglobin Constant Spring--a chain termination mutant? Nature. 1971 Dec 10;234(5328):337–340. doi: 10.1038/234337a0. [DOI] [PubMed] [Google Scholar]
  2. Efstratiadis A., Kafatos F. C., Maniatis T. The primary structure of rabbit beta-globin mRNA as determined from cloned DNA. Cell. 1977 Apr;10(4):571–585. doi: 10.1016/0092-8674(77)90090-3. [DOI] [PubMed] [Google Scholar]
  3. Forget B. G., Hillman D. G., Lazarus H., Barell E. F., Benz ej J. R., Caskey C. T., Huisman T. H., Schroeder W. A., Housman D. Absence of messenger RNA and gene DNA for beta-globin chains in hereditary persistence of fetal hemoglobin. Cell. 1976 Mar;7(3):323–329. doi: 10.1016/0092-8674(76)90161-6. [DOI] [PubMed] [Google Scholar]
  4. Forget B. G., Marotta C. A., Weissman S. M., Verma I. M., McCaffrey R. P., Baltimore D. Nucleotide sequences of human globin messenger RNA. Ann N Y Acad Sci. 1974 Nov 29;241(0):290–309. doi: 10.1111/j.1749-6632.1974.tb21888.x. [DOI] [PubMed] [Google Scholar]
  5. Galibert F., Sedat J., Ziff E. Direct determination of DNA nucleotide sequences: structure of a fragment of bacteriophage phiX172 DNA. J Mol Biol. 1974 Aug 15;87(3):377–407. doi: 10.1016/0022-2836(74)90093-x. [DOI] [PubMed] [Google Scholar]
  6. Kan Y. W., Todd D., Dozy A. M. Haemoglobin Constant Spring synthesis in red cell precursors. Br J Haematol. 1974 Sep;28(1):103–107. doi: 10.1111/j.1365-2141.1974.tb06643.x. [DOI] [PubMed] [Google Scholar]
  7. Maniatis T., Jeffrey A., Kleid D. G. Nucleotide sequence of the rightward operator of phage lambda. Proc Natl Acad Sci U S A. 1975 Mar;72(3):1184–1188. doi: 10.1073/pnas.72.3.1184. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Marotta C. A., Lebowitz P., Dhar R., Zain B. S., Weissman S. M. Preparation of RNA transcripts of discrete segments of DNA. Methods Enzymol. 1974;29:254–272. doi: 10.1016/0076-6879(74)29026-8. [DOI] [PubMed] [Google Scholar]
  9. Proudfoot N. J., Brownlee G. G. 3' non-coding region sequences in eukaryotic messenger RNA. Nature. 1976 Sep 16;263(5574):211–214. doi: 10.1038/263211a0. [DOI] [PubMed] [Google Scholar]
  10. Proudfoot N. J. Complete 3' noncoding region sequences of rabbit and human beta-globin messenger RNAs. Cell. 1977 Apr;10(4):559–570. doi: 10.1016/0092-8674(77)90089-7. [DOI] [PubMed] [Google Scholar]
  11. Proudfoot N. J., Longley J. I. The 3' terminal sequences of human alpha and beta globin messenger RNAs: comparison with rabbit globin messenger RNA. Cell. 1976 Dec;9(4 Pt 2):733–746. doi: 10.1016/0092-8674(76)90137-9. [DOI] [PubMed] [Google Scholar]
  12. Proudfoot N. J. Sequence analysis of the 3' non-coding regions of rabbit alpha- and beta-globin messenger RNAs. J Mol Biol. 1976 Nov 15;107(4):491–525. doi: 10.1016/s0022-2836(76)80080-0. [DOI] [PubMed] [Google Scholar]
  13. Richardson C. C. Phosphorylation of nucleic acid by an enzyme from T4 bacteriophage-infected Escherichia coli. Proc Natl Acad Sci U S A. 1965 Jul;54(1):158–165. doi: 10.1073/pnas.54.1.158. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Seid-Akhavan M., Winter W. P., Abramson R. K., Rucknagel D. L. Hemoglobin Wayne: a frameshift mutation detected in human hemoglobin alpha chains. Proc Natl Acad Sci U S A. 1976 Mar;73(3):882–886. doi: 10.1073/pnas.73.3.882. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Sharp P. A., Sugden B., Sambrook J. Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis. Biochemistry. 1973 Jul 31;12(16):3055–3063. doi: 10.1021/bi00740a018. [DOI] [PubMed] [Google Scholar]
  16. Smith H. O., Wilcox K. W. A restriction enzyme from Hemophilus influenzae. I. Purification and general properties. J Mol Biol. 1970 Jul 28;51(2):379–391. doi: 10.1016/0022-2836(70)90149-x. [DOI] [PubMed] [Google Scholar]
  17. Subramanian K. N., Pan J., Zain S., Weissman S. M. The mapping and ordering of fragments of SV40 DNA produced by restriction endonucleases. Nucleic Acids Res. 1974 Jun;1(6):727–752. doi: 10.1093/nar/1.6.727. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Weatherall D. J., Clegg J. B. The alpha-chain-termination mutants and their relation to the alpha-thalassaemias. Philos Trans R Soc Lond B Biol Sci. 1975 Aug 7;271(913):411–455. doi: 10.1098/rstb.1975.0061. [DOI] [PubMed] [Google Scholar]
  19. Wilson J. T., Forget B. G., Wilson L. B., Weissman S. M. Human globin messenger RNA: importance of cloning for structural analysis. Science. 1977 Apr 8;196(4286):200–202. doi: 10.1126/science.847468. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES