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. 1977 Aug;4(8):2561–2572. doi: 10.1093/nar/4.8.2561

A simple, general procedure for purifying restriction endonucleases.

T A Bickle, V Pirrotta, R Imber
PMCID: PMC342592  PMID: 909783

Abstract

A simple, general method for purifying restriction endonucleases is described. The method employs precipitation of nucleic acids from crude extracts with polyethyleneimine followed by affinity chromatography on columns of heparin covalently linked to agarose. Most of the sixteen enzymes tested could be purified to a degree sufficient for DNA sequencing work by this method sometimes supplemented by at most one step of ion exchange chromatography.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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