Skip to main content
PMC home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1977 Aug;4(8):2671–2682. doi: 10.1093/nar/4.8.2671

Methylated constituents of Aedes albopictus poly (A)-containing messenger RNA.

C C HsuChen, D T Dubin
PMCID: PMC342600  PMID: 198748

Abstract

Poly (A)-containing mRNA prepared from cultured mosquito (Aedes albopictus) cells was found to contain methylated 5'-terminal "caps" as well as internal m6A residues. Both type I [m7G(5')ppp(5')Xmp] and type II [m7G(5')ppp(5')XmpYmp] caps were present, at molar ratio of ca five to one. All four common RNA bases were represented in the second position (Xm) of the caps, adenine being the most abundant and N6-methyladenine being absent. The four bases were also represented in the third position (Ym), but here uracil was the predominant base. There was approximately one internal m6A residue for every three caps. These studies demonstrate that mRNA from an invertebrate source can have a methylation pattern comparable with that of mammalian cells in it complexity.

Full text

PDF
2677

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cory S., Adams J. M. The modified 5'-terminal sequences in messenger RNA of mouse myeloma cells. J Mol Biol. 1975 Dec 25;99(4):519–547. doi: 10.1016/s0022-2836(75)80170-7. [DOI] [PubMed] [Google Scholar]
  2. DUBIN D. T. SOME EFFECT OF STREPTOMYCIN ON RNA METABOLISM IN ESCHERICHIA COLI. J Mol Biol. 1964 May;8:749–767. doi: 10.1016/s0022-2836(64)80122-4. [DOI] [PubMed] [Google Scholar]
  3. DULBECCO R., VOGT M. One-step growth curve of Western equine encephalomyelitis virus on chicken embryo cells grown in vitro and analysis of virus yields from single cells. J Exp Med. 1954 Feb;99(2):183–199. doi: 10.1084/jem.99.2.183. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Desrosiers R. C., Friderici K. H., Rottman F. M. Characterization of Novikoff hepatoma mRNA methylation and heterogeneity in the methylated 5' terminus. Biochemistry. 1975 Oct 7;14(20):4367–4374. doi: 10.1021/bi00691a004. [DOI] [PubMed] [Google Scholar]
  5. Dottin R. P., Weiner A. M., Lodish F. 5' terminal nucleotide sequences of the messenger RNA's of Dictyostelium discoideum. Cell. 1976 Jun;8(2):233–244. doi: 10.1016/0092-8674(76)90007-6. [DOI] [PubMed] [Google Scholar]
  6. Dubin D. T. Methylated nucleotide content of mitochondrial ribosomal RNA from hamster cells. J Mol Biol. 1974 Apr 5;84(2):257–273. doi: 10.1016/0022-2836(74)90584-1. [DOI] [PubMed] [Google Scholar]
  7. Dubin D. T., Taylor R. H. The methylation state of poly A-containing messenger RNA from cultured hamster cells. Nucleic Acids Res. 1975 Oct;2(10):1653–1668. doi: 10.1093/nar/2.10.1653. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. EAGLE H. Amino acid metabolism in mammalian cell cultures. Science. 1959 Aug 21;130(3373):432–437. doi: 10.1126/science.130.3373.432. [DOI] [PubMed] [Google Scholar]
  9. Faust M., Millward S., Duchastel A., Fromson D. Methylated constituents of poly(A)- and poly(A)+ polyribosomal RNA of sea urchin embryos. Cell. 1976 Dec;9(4 Pt 1):597–604. doi: 10.1016/0092-8674(76)90042-8. [DOI] [PubMed] [Google Scholar]
  10. Groner Y., Grosfeld H., Littauer U. Z. 5'-Capping structures of Artemia salina mRNA and the translational inhibition by cap analogs. Eur J Biochem. 1976 Dec;71(1):281–293. doi: 10.1111/j.1432-1033.1976.tb11114.x. [DOI] [PubMed] [Google Scholar]
  11. Kastern W. H., Berry S. J. Non-methylated guanosine as the 5' terminus of capped mRNA from insect oocytes. Biochem Biophys Res Commun. 1976 Jul 12;71(1):37–44. doi: 10.1016/0006-291x(76)90246-1. [DOI] [PubMed] [Google Scholar]
  12. Lengyel J., Penman S. hnRNA size and processing as related to different DNA content in two dipterans: Drosophila and Aedes. Cell. 1975 Jul;5(3):281–290. doi: 10.1016/0092-8674(75)90103-8. [DOI] [PubMed] [Google Scholar]
  13. Mager W. H., Klootwijk J., Klein I. Minimal methylation of yeast messenger RNA. Mol Biol Rep. 1976 Sep;3(1):9–17. doi: 10.1007/BF00357204. [DOI] [PubMed] [Google Scholar]
  14. Perry R. P., Kelley D. E. Kinetics of formation of 5' terminal caps in mRNA. Cell. 1976 Jul;8(3):433–442. doi: 10.1016/0092-8674(76)90156-2. [DOI] [PubMed] [Google Scholar]
  15. Salditt-Georgieff M., Jelinek W., Darnell J. E., Furuichi Y., Morgan M., Shatkin A. Methyl labeling of HeLa cell hnRNA: a comparison with mRNA. Cell. 1976 Feb;7(2):227–237. doi: 10.1016/0092-8674(76)90022-2. [DOI] [PubMed] [Google Scholar]
  16. Shatkin A. J. Capping of eucaryotic mRNAs. Cell. 1976 Dec;9(4 Pt 2):645–653. doi: 10.1016/0092-8674(76)90128-8. [DOI] [PubMed] [Google Scholar]
  17. Spradling A., Hui H., Penman S. Two very different components of messenger RNA in an insect cell line. Cell. 1975 Feb;4(2):131–137. doi: 10.1016/0092-8674(75)90119-1. [DOI] [PubMed] [Google Scholar]
  18. Sripati C. E., Groner Y., Warner J. R. Methylated, blocked 5' termini of yeast mRNA. J Biol Chem. 1976 May 25;251(10):2898–2904. [PubMed] [Google Scholar]
  19. Surrey S., Nemer M. Methylated blocked 5' terminal sequences of sea urchin embryo messenger RNA classes containing and lacking poly(A). Cell. 1976 Dec;9(4 Pt 1):589–595. doi: 10.1016/0092-8674(76)90041-6. [DOI] [PubMed] [Google Scholar]
  20. Wei C. M., Gershowitz A., Moss B. 5'-Terminal and internal methylated nucleotide sequences in HeLa cell mRNA. Biochemistry. 1976 Jan 27;15(2):397–401. doi: 10.1021/bi00647a024. [DOI] [PubMed] [Google Scholar]
  21. Wei C. M., Moss B. Methylated nucleotides block 5'-terminus of vaccinia virus messenger RNA. Proc Natl Acad Sci U S A. 1975 Jan;72(1):318–322. doi: 10.1073/pnas.72.1.318. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Yang N. S., Manning R. F., Gage L. P. The blocked and methylated 5' terminal sequence of a specific cellular messenger: the mRNA for silk fibroin of Bombyx mori. Cell. 1976 Mar;7(3):339–347. doi: 10.1016/0092-8674(76)90163-x. [DOI] [PubMed] [Google Scholar]
  23. al-Arif A., Sporn M. B. An analytical method for the separation of sugar-methylated ribonucleosides from base-methylated and nonmethylated ribonucleosides. Anal Biochem. 1972 Aug;48(2):386–393. doi: 10.1016/0003-2697(72)90091-7. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES