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. 1977 Sep;4(9):3017–3027. doi: 10.1093/nar/4.9.3017

Changes in chromatin structure induced by EDTA treatment and partial removal of histone H1.

Y Y Vengerov, V I Popenko
PMCID: PMC342631  PMID: 410005

Abstract

Electron microscopy shows that EDTA treatment or partial removal of histone HI converts 200-250 A chromatin fibres characteristic for native chromatin, isolated in low ionic strength conditions into fibres consisting of nucleosomes connected by segments of DNA. This structural transition is accompanied by an increase in the amplitude of positive band of CD spectra at 280 nm. Comparison of electron microscopic, thermal denaturation and electrophoretic data suggests that multiphasic character of melting curves, observed for chromatin, lacking histone HI is due to the removal of histone HI and destabilisation of the DNA segments, connecting nucleosomes. It is also shown that bivalent cations play an important part both in the stabilisation of 200 A globules and of nucleosomes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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