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. 1977 Sep;4(9):3187–3198. doi: 10.1093/nar/4.9.3187

Purification of cDNA complementary to sea urchin histone mRNA.

D Woods, W Fitschen
PMCID: PMC342643  PMID: 909803

Abstract

Complementary DNA (cDNA) was transcribed from a polyadenylated sea urchin histone mRNA preparation isolated by density gradient centrifugation. By hybridization, this cDNA was shown to be extensively contaminated (85% of hybridizable cDNA) with DNA complementary to RNA derived from the large ribosomal subunit. Purification of a mRNA specific cDNA fraction was achieved by hybridization of purified rRNA to cDNA followed by fractionation on hydroxyapatite. After further purification to remove nonhybridizable cDNA our purified cDNA showed only 8% hybirdization to rRNA.

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Selected References

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