Skip to main content
PMC home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1975 Jan;2(1):61–78. doi: 10.1093/nar/2.1.61

Formylatable methionine transfer RNA from Mycoplasma: purification and comparison of partial nucleotide sequences with those of other prokaryotic initiator tRNAs.

R T Walker, U L RajBhandary
PMCID: PMC342811  PMID: 1093144

Abstract

The major species of the formylatable methionine tRNA from Mycoplasma mycoides var capri has been purified. The 5'- and 3'-terminal sequences of the purified tRNA are pC-G- and C-A-A-C-C-AOH, respectively. Thus, this tRNA also contains the unique structural feature found in two other prokaryotic initiator tRNAs in that the first nucleotide at the 5'-end cannot form a Watson-Crick type of base-pair to the fifth nucleotide from the 3'-end. The Mycoplasma tRNA does not contain ribothymidine; however, a specific uridine residue in the sequence G-U-psi-C-G- can be enzymatically methylated by E. coli extracts to yield G-T-psi-C-G. Since ribothymidine is absent in crude tRNA from this strain of Mycoplasma, the absence of T is probably due to the lack of a U yields T modifying enzyme.

Full text

PDF
66

Images in this article

70AImage
on p.70A
70EImage
on p.70E
70BImage
on p.70B
70BImage
on p.70B
70CImage
on p.70C
70DImage
on p.70D
72AImage
on p.72A
74AImage
on p.74A

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Dube S. K., Marcker K. A., Clark B. F., Cory S. Nucleotide sequence of N-formyl-methionyl-transfer RNA. Nature. 1968 Apr 20;218(5138):232–233. doi: 10.1038/218232a0. [DOI] [PubMed] [Google Scholar]
  2. Ecarot B., Cedergren R. J. Structure-function correlations of formylmethionine transfer RNA. Biochem Biophys Res Commun. 1974 Jul 10;59(1):400–405. doi: 10.1016/s0006-291x(74)80220-2. [DOI] [PubMed] [Google Scholar]
  3. Egan B. Z. Separation of oligonucleotides by reversed-phase chromatography. Biochim Biophys Acta. 1973 Mar 19;299(2):245–252. doi: 10.1016/0005-2787(73)90347-x. [DOI] [PubMed] [Google Scholar]
  4. Feldmann H., Falter H. Transfer ribonucleic acid from Mycoplasma laidlawii A. Eur J Biochem. 1971 Feb;18(4):573–581. doi: 10.1111/j.1432-1033.1971.tb01278.x. [DOI] [PubMed] [Google Scholar]
  5. Ghosh K., Ghosh H. P., Simsek M., Raj Bhandary U. L. Initiator methionine transfer ribonucleic acid from wheat embryo. Purification, properties, and partial nucleotide sequences. J Biol Chem. 1974 Aug 10;249(15):4720–4729. [PubMed] [Google Scholar]
  6. Hayashi H., Fisher H., Söll D. Transfer ribonucleic acid from Mycoplasma. Biochemistry. 1969 Sep;8(9):3680–3686. doi: 10.1021/bi00837a028. [DOI] [PubMed] [Google Scholar]
  7. JONES A. S., WALKER R. T. Isolation and analysis of the deoxyribonucleic acid of Mycoplasma mycoides var. Capri. Nature. 1963 May 11;198:588–589. doi: 10.1038/198588a0. [DOI] [PubMed] [Google Scholar]
  8. Johnson L., Hayashi H., Söll D. Isolation and properties of a transfer ribonucleic acid deficient in ribothymidine. Biochemistry. 1970 Jul 7;9(14):2823–2831. doi: 10.1021/bi00816a011. [DOI] [PubMed] [Google Scholar]
  9. Jones A. S., Tittensor J. R., Walker R. T. The chemical composition of the nucleic acids and other macromolecular constituents of Mycoplasma mycoides var. capri. J Gen Microbiol. 1965 Sep;40(3):405–411. doi: 10.1099/00221287-40-3-405. [DOI] [PubMed] [Google Scholar]
  10. Kelmers A. D., Heatherly D. E. Columns for rapid chromatographic separation of small amounts of tracer-labeled transfer ribonucleic acids. Anal Biochem. 1971 Dec;44(2):486–495. doi: 10.1016/0003-2697(71)90236-3. [DOI] [PubMed] [Google Scholar]
  11. Kössel H., RajBhandary U. L. Studies on polynucleotides. LXXXVI. Enzymic hydrolysis of N-acylaminoacyl-transfer RNA. J Mol Biol. 1968 Aug 14;35(3):539–560. doi: 10.1016/s0022-2836(68)80013-0. [DOI] [PubMed] [Google Scholar]
  12. Nishimura S., Harada F., Narushima U., Seno T. Purification of methionine-, valine-, phenylalanine- and tyrosine-specific tRNA from Escherichia coli. Biochim Biophys Acta. 1967 Jun 20;142(1):133–148. doi: 10.1016/0005-2787(67)90522-9. [DOI] [PubMed] [Google Scholar]
  13. Ono Y., Skoultchi A., Klein A., Lengyel P. Peptide chain elongation: discrimination against the initiator transfer RNA by microbial amino-acid polymerization factors. Nature. 1968 Dec 28;220(5174):1304–1307. doi: 10.1038/2201304a0. [DOI] [PubMed] [Google Scholar]
  14. Pearson R. L., Weiss J. F., Kelmers A. D. Improved separation of transfer RNA's on polychlorotrifuoroethylene-supported reversed-phase chromatography columns. Biochim Biophys Acta. 1971 Feb 11;228(3):770–774. doi: 10.1016/0005-2787(71)90748-9. [DOI] [PubMed] [Google Scholar]
  15. Petrissant G. Evidence for the absence of the G-T-psi-C sequence from two mammalian initiator transfer RNAs. Proc Natl Acad Sci U S A. 1973 Apr;70(4):1046–1049. doi: 10.1073/pnas.70.4.1046. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Piper P. W., Clark B. F. Lack of Tp-psi-p in loop IV of a mammalian initiator transfer RNA. FEBS Lett. 1973 Mar 15;30(3):265–267. doi: 10.1016/0014-5793(73)80666-0. [DOI] [PubMed] [Google Scholar]
  17. Piper P. W., Clark B. F. Primary structure of a mouse myeloma cell initiator transfer RNA. Nature. 1974 Feb 22;247(5442):516–518. doi: 10.1038/247516a0. [DOI] [PubMed] [Google Scholar]
  18. RajBhandary U. L., Ghosh H. P. Studies on polynucleotides. XCI. Yeast methionine transfer ribonucleic acid: purification, properties, and terminal nucleotide sequences. J Biol Chem. 1969 Mar 10;244(5):1104–1113. [PubMed] [Google Scholar]
  19. Sanger F., Brownlee G. G., Barrell B. G. A two-dimensional fractionation procedure for radioactive nucleotides. J Mol Biol. 1965 Sep;13(2):373–398. doi: 10.1016/s0022-2836(65)80104-8. [DOI] [PubMed] [Google Scholar]
  20. Schulman L. H., Her M. O. Recognition of altered E. coli formylmethionine transfer RNA by bacterial T factor. Biochem Biophys Res Commun. 1973 Mar 17;51(2):275–282. doi: 10.1016/0006-291x(73)91253-9. [DOI] [PubMed] [Google Scholar]
  21. Simsek M., Petrissant G., Rajbhandary U. L. Replacement of the sequence G-T-phi-C-G(A)- by G-A-U-C-G- in initiator transfer RNA of rabbit-liver cytoplasm. Proc Natl Acad Sci U S A. 1973 Sep;70(9):2600–2604. doi: 10.1073/pnas.70.9.2600. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Simsek M., RajBhandary U. L., Boisnard M., Petrissant G. Nucleotide sequence of rabbit liver and sheep mammary gland cytoplasmic initiatory transfer RNAs. Nature. 1974 Feb 22;247(5442):518–520. doi: 10.1038/247518a0. [DOI] [PubMed] [Google Scholar]
  23. Simsek M., RajBhandary U. L. The primary structure of yeast initiator transfer ribonucleic acid. Biochem Biophys Res Commun. 1972 Oct 17;49(2):508–515. doi: 10.1016/0006-291x(72)90440-8. [DOI] [PubMed] [Google Scholar]
  24. Simsek M., Ziegenmeyer J., Heckman J., Rajbhandary U. L. Absence of the sequence G-T-psi-C-G(A)- in several eukaryotic cytoplasmic initiator transfer RNAs. Proc Natl Acad Sci U S A. 1973 Apr;70(4):1041–1045. doi: 10.1073/pnas.70.4.1041. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Vogel Z., Zamir A., Elson D. The specific resistance of N-substituted initiator methionyl-+RNA to enzymatic hydrolysis. Biochem Biophys Res Commun. 1968 Oct 10;33(1):94–98. doi: 10.1016/0006-291x(68)90260-x. [DOI] [PubMed] [Google Scholar]
  26. Walker R. T. DNA homology of two Mycoplasma species. Nature. 1967 Nov 18;216(5116):711–712. doi: 10.1038/216711a0. [DOI] [PubMed] [Google Scholar]
  27. Walker R. T., RajBhandary U. L. Studies on polynucleotides. CI. Escherichia coli tyrosine and formylmethionine transfer ribonucleic acids: effect of chemical modification of 4-thiouridine to uridine on their biological properties. J Biol Chem. 1972 Aug 10;247(15):4879–4892. [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES