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. 1976 Feb;3(2):343–353. doi: 10.1093/nar/3.2.343

The isolation and partial characterization of a new restriction endonuclease from Providencia stuartii.

D I Smith, F R Blattner, J Davies
PMCID: PMC342905  PMID: 768923

Abstract

We describe the isolation of a new class 2 restriction endonuclease from Providencia stuartii 164. Using the procedure of osmotic shock treatment, we have partially purified this enzyme (Pst 1) and have begun preliminary work to characterize its specificity and requirements. Pst 1 requires Mg++ as the only cofactor and produces more than 18 cleavages in wild type lambda. We have determined the location of 7 of these cleavages by the use of deletion and insertion mutants of lambda.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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