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. 1976 May;3(5):1323–1339. doi: 10.1093/nar/3.5.1323

A restriction cleavage map of øX 174 DNA by pulse-chase labelling using E.coli DNA polymerase

Peter GN Jeppesen 1, Louise Sanders 1, Patrick M Slocombe 1
PMCID: PMC342989  PMID: 781625

Abstract

A pulse chase labelling technique using E. coli DNA polymerase I has been used to determine a number of restriction endonuclease cleavage maps of ØX 174 DNA. In addition to verifying the accuracy of the method by confirming some previously published maps, the Hha I and Alu I maps have also been constructed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Air G. M., Blackburn E. H., Sanger F., Coulson A. R. The nucleotide and amino acid sequences of the N (5') terminal region of gene G of bacteriophage phiphiX 174. J Mol Biol. 1975 Aug 25;96(4):703–719. doi: 10.1016/0022-2836(75)90147-3. [DOI] [PubMed] [Google Scholar]
  2. Allet B., Jeppesen P. G., Katagiri K. J., Delius H. Mapping the DNA fragments produced by cleavage by lambda DNA with endonuclease RI. Nature. 1973 Jan 12;241(5385):120–123. doi: 10.1038/241120a0. [DOI] [PubMed] [Google Scholar]
  3. Brown W. M., Vinograd J. Restriction endonuclease cleavage maps of animal mitochondrial DNAs. Proc Natl Acad Sci U S A. 1974 Nov;71(11):4617–4621. doi: 10.1073/pnas.71.11.4617. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chen C. Y., Hutchison C. A., 3rd, Edgell M. H. Isolation and genetic localization of three phi-X174 promoter regions. Nat New Biol. 1973 Jun 20;243(129):233–236. doi: 10.1038/newbio243233a0. [DOI] [PubMed] [Google Scholar]
  5. Danna K. J., Sack G. H., Jr, Nathans D. Studies of simian virus 40 DNA. VII. A cleavage map of the SV40 genome. J Mol Biol. 1973 Aug 5;78(2):363–376. doi: 10.1016/0022-2836(73)90122-8. [DOI] [PubMed] [Google Scholar]
  6. Edgell M. H., Hutchison C. A., 3rd, Sclair M. Specific endonuclease R fragments of bacteriophage phiX174 deoxyribonucleic acid. J Virol. 1972 Apr;9(4):574–582. doi: 10.1128/jvi.9.4.574-582.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Garfin D. E., Goodman H. M. Nucleotide sequences at the cleavage sites of two restriction endonucleases from Hemophilus parainfluenzae. Biochem Biophys Res Commun. 1974 Jul 10;59(1):108–116. doi: 10.1016/s0006-291x(74)80181-6. [DOI] [PubMed] [Google Scholar]
  8. Griffin B. E., Fried M., Cowie A. Polyoma DNA: a physical map. Proc Natl Acad Sci U S A. 1974 May;71(5):2077–2081. doi: 10.1073/pnas.71.5.2077. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hayashi M. N., Hayashi M. Fragment maps of phiX-174 replicative DNA produced by restriction enzymes from haemophilus aphirophilus and haemophilus influenzae H-I. J Virol. 1974 Nov;14(5):1142–1151. doi: 10.1128/jvi.14.5.1142-1151.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Jeppesen P. G. A method for separating DNA fragments by electrophoresis in polyacrylamide concentration gradient slab gels. Anal Biochem. 1974 Mar;58(1):195–207. doi: 10.1016/0003-2697(74)90458-8. [DOI] [PubMed] [Google Scholar]
  11. Johnson P. H., Sinsheimer R. L. Structure of an intermediate in the replication of bacteriophage phiX174 deoxyribonucleic acid: the initiation site for DNA replication. J Mol Biol. 1974 Feb 15;83(1):47–61. doi: 10.1016/0022-2836(74)90423-9. [DOI] [PubMed] [Google Scholar]
  12. Kelly T. J., Jr, Smith H. O. A restriction enzyme from Hemophilus influenzae. II. J Mol Biol. 1970 Jul 28;51(2):393–409. doi: 10.1016/0022-2836(70)90150-6. [DOI] [PubMed] [Google Scholar]
  13. Lee A. S., Sinsheimer R. L. A cleavage map of bacteriophage phiX174 genome. Proc Natl Acad Sci U S A. 1974 Jul;71(7):2882–2886. doi: 10.1073/pnas.71.7.2882. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Maniatis T., Jeffrey A., Kleid D. G. Nucleotide sequence of the rightward operator of phage lambda. Proc Natl Acad Sci U S A. 1975 Mar;72(3):1184–1188. doi: 10.1073/pnas.72.3.1184. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Middleton J. H., Edgell M. H., Hutchison C. A., 3rd Specific fragments of phi X174 deoxyribonucleic acid produced by a restriction enzyme from Haemophilus aegyptius, endonuclease Z. J Virol. 1972 Jul;10(1):42–50. doi: 10.1128/jvi.10.1.42-50.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Mulder C., Arrand J. R., Delius H., Keller W., Pettersson U., Roberts R. J., Sharp P. A. Cleavage maps of DNA from adenovirus types 2 and 5 by restriction endonucleases EcoRI and HpaI. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 1):397–400. doi: 10.1101/sqb.1974.039.01.051. [DOI] [PubMed] [Google Scholar]
  17. Sanger F., Coulson A. R. A rapid method for determining sequences in DNA by primed synthesis with DNA polymerase. J Mol Biol. 1975 May 25;94(3):441–448. doi: 10.1016/0022-2836(75)90213-2. [DOI] [PubMed] [Google Scholar]
  18. Smith H. O., Wilcox K. W. A restriction enzyme from Hemophilus influenzae. I. Purification and general properties. J Mol Biol. 1970 Jul 28;51(2):379–391. doi: 10.1016/0022-2836(70)90149-x. [DOI] [PubMed] [Google Scholar]
  19. Sugisaki H., Takanami M. DNA sequence restricted by restriction endonuclease AP from Haemophilus aphirophilus. Nat New Biol. 1973 Dec 5;246(153):138–140. doi: 10.1038/newbio246138a0. [DOI] [PubMed] [Google Scholar]
  20. Summers J. Physical map of polyoma viral DNA fragments produced by cleavage with a restriction enzyme from Haemophilus aegyptius, endonuclease R-HaeIII. J Virol. 1975 Apr;15(4):946–953. doi: 10.1128/jvi.15.4.946-953.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Vereijken J. M., Van Mansfeld A. D., Baas P. D., Jansz H. S. Arthrobacter luteus restriction endonuclease cleavage map of phi chi 174 RF DNA. Virology. 1975 Nov;68(1):221–233. doi: 10.1016/0042-6822(75)90163-4. [DOI] [PubMed] [Google Scholar]

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