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. 1976 Aug;3(8):2089–2099. doi: 10.1093/nar/3.8.2089

Polynucleotides. XL. Synthesis and properties of poly 2'-azido-2'-deoxyadenylic acid.

M Ikehara, T Fukui, N Kakiuchi
PMCID: PMC343064  PMID: 9620

Abstract

Poly 2'-azido-2'-deoxyadenylic acid (Poly Az) was synthesized from 2'-azido-2'-deoxyadenosine diphosphate by polynucleotide phosphorylase. Poly (Az) has U.V. absorption properties similar to poly (A) and hypochromicity of 40% at 0.1 M Na+ and neutrality. CD curve also resembled to that of poly (A), but has smaller ellipticity. Titration of poly (Az) with HCl gave a transition at pH 5.5, but exact structure of the acid-form complex was not elucidated. Upon mixing with poly (U), poly (Az) forms a 1:1 and 1:2 complexes having Tm's somewhat higher than that of poly (A)- poly (U) complex in the same condition.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alderfer J. L., Tazawa I., Tazawa S., Ts'o P. Comparative studies on homopolymers of adenylic acid possessing different C-2' substituents of the furanose. Poly(deoxyriboadenylic acid), poly(riboadenylic acid),poly(2'-O-methyladenylic acid), and poly(2'-O-ethyladenylic acid). Biochemistry. 1974 Apr 9;13(8):1615–1622. doi: 10.1021/bi00705a010. [DOI] [PubMed] [Google Scholar]
  2. Blake R. D., Massoulié J., Fresco J. R. Polynucleotides. 8. A spectral approach to the equilibria between polyriboadenylate and polyribouridylate and their complexes. J Mol Biol. 1967 Dec 14;30(2):291–308. [PubMed] [Google Scholar]
  3. Bobst A. M., Rottman F., Cerutti P. A. Effect of the methylation of the 2'-hydroxyl groups in polyadenylic acid on its structure in weakly acidic and neutral solutions and on its capability to form ordered complexes with polyuridylic acid. J Mol Biol. 1969 Dec 14;46(2):221–234. doi: 10.1016/0022-2836(69)90418-5. [DOI] [PubMed] [Google Scholar]
  4. Cassani G. R., Bollum F. J. Oligodeoxythymidylate: polydeoxyadenylate and oligodeoxyadenylate: polydeoxythymidylate interactions. Biochemistry. 1969 Oct;8(10):3928–3936. doi: 10.1021/bi00838a008. [DOI] [PubMed] [Google Scholar]
  5. FELSENFELD G., RICH A. Studies on the formation of two- and three-stranded polyribonucleotides. Biochim Biophys Acta. 1957 Dec;26(3):457–468. doi: 10.1016/0006-3002(57)90091-4. [DOI] [PubMed] [Google Scholar]
  6. Hobbs J., Sternbach H., Sprinzl M., Eckstein F. Polynucleotides containing 2'-amino-2'-deoxyribose and 2'-azido-2'-deoxyribose. Biochemistry. 1973 Dec 4;12(25):5138–5145. doi: 10.1021/bi00749a018. [DOI] [PubMed] [Google Scholar]
  7. Hobbs J., Sternbach H., Sprinzl M., Eckstein F. Polynucleotides containing 2'-chloro-2'-deoxyribose. Biochemistry. 1972 Nov 7;11(23):4336–4344. doi: 10.1021/bi00773a021. [DOI] [PubMed] [Google Scholar]
  8. Ikehara M., Hattori M., Fukui T. Synthesis and properties of poly(2-methyladenylic acid). Formation of a poly(A)-poly(U) complex with Hoogsteen-type hydrogen binding. Eur J Biochem. 1972 Dec 4;31(2):329–334. doi: 10.1111/j.1432-1033.1972.tb02537.x. [DOI] [PubMed] [Google Scholar]
  9. Janik B., Kotick M. P., Kreiser T. H., Reverman L. F., Sommer R. G., Wilson D. P. Synthesis and properties of poly 2'-fluoro-2'-deoxyuridylic acid. Biochem Biophys Res Commun. 1972 Feb 16;46(3):1153–1160. doi: 10.1016/s0006-291x(72)80095-0. [DOI] [PubMed] [Google Scholar]
  10. Kuśmierek J. T., Kielanowska M., Shugar D. Preparation and stability of the helical form of poly 2'-O-ethyluridylic acid. Biochem Biophys Res Commun. 1973 Jul 17;53(2):406–412. doi: 10.1016/0006-291x(73)90676-1. [DOI] [PubMed] [Google Scholar]
  11. Michelson A. M., Massoulié J., Guschlbauer W. Synthetic polynucleotides. Prog Nucleic Acid Res Mol Biol. 1967;6:83–141. doi: 10.1016/s0079-6603(08)60525-5. [DOI] [PubMed] [Google Scholar]
  12. RICH A., DAVIES D. R., CRICK F. H., WATSON J. D. The molecular structure of polyadenylic acid. J Mol Biol. 1961 Feb;3:71–86. doi: 10.1016/s0022-2836(61)80009-0. [DOI] [PubMed] [Google Scholar]
  13. Torrence P. F., Bobst A. M., Waters J. A., Witkop B. Synthesis and characterization of potential interferon inducers. Poly(2'-azido-2'-deoxyuridylic acid). Biochemistry. 1973 Sep 25;12(20):3962–3972. doi: 10.1021/bi00744a028. [DOI] [PubMed] [Google Scholar]
  14. Van Holde K. E., Brahms J., Michelson A. M. Base interactions of nucleotide polymers in aqueous solution. J Mol Biol. 1965 Jul;12(3):726–739. doi: 10.1016/s0022-2836(65)80323-0. [DOI] [PubMed] [Google Scholar]
  15. WILLIAMS F. R., GRUNBERG-MANAGO M. POLYNUCL'EOTIDE PHOSPHORYLASE HAUTEMENT PURIFI'EE 'A PARTIR DE E. COLI. Biochim Biophys Acta. 1964 Jul 8;89:66–89. [PubMed] [Google Scholar]
  16. Zmudzka B., Shugar D. Role of the 2'-hydroxyl in polynucleotide conformation: poly 2'-O-methyluridylic acid. Acta Biochim Pol. 1971;18(3):321–337. [PubMed] [Google Scholar]

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