Skip to main content
PMC home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1976 Oct;3(10):2427–2436. doi: 10.1093/nar/3.10.2427

The 5' ends of yeast killer factor RNAs are pppGp.

J Bruenn, B Keitz
PMCID: PMC343101  PMID: 792814

Abstract

The 5' nucleotides of the double-stranded RNAs of yeast killer factor have been isolated by digestion with pancreatic, T1 and T2 RNase followed by two-dimensional electrophoresis. They were identified by bacterial alkaline phosphatase and snake venom phosphodiesterase digestions. Both the larger double-stranded RNA (L, of 2.5 x 10(6) daltons) and the smaller double-stranded RNA (M, of 1.4 x 10(6) daltons) have the 5' end groups pppGp. These 5' ends are dissimilar to those of the double-stranded RNAs of animal viruses but may be characteristic of the 5' ends of the double-stranded RNAs of fungal viruses.

Full text

PDF
2431

Images in this article

2430Image
on p.2430
2433Image
on p.2433
2434Image
on p.2434

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adams J. M., Cory S. Modified nucleosides and bizarre 5'-termini in mouse myeloma mRNA. Nature. 1975 May 1;255(5503):28–33. doi: 10.1038/255028a0. [DOI] [PubMed] [Google Scholar]
  2. Adler J., Wood H. A., Bozarth R. F. Virus-like particles from killer, neutral, and sensitive strains of Saccharomyces cerevisiae. J Virol. 1976 Feb;17(2):472–476. doi: 10.1128/jvi.17.2.472-476.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bevan E. A., Herring A. J., Mitchell D. J. Preliminary characterization of two species of dsRNA in yeast and their relationship to the "killer" character. Nature. 1973 Sep 14;245(5420):81–86. doi: 10.1038/245081b0. [DOI] [PubMed] [Google Scholar]
  4. Both G. W., Furuichi Y., Muthukrishnan S., Shatkin A. J. Ribosome binding to reovirus mRNA in protein synthesis requires 5' terminal 7-methylguanosine. Cell. 1975 Oct;6(2):185–195. doi: 10.1016/0092-8674(75)90009-4. [DOI] [PubMed] [Google Scholar]
  5. Buck K. W. Replication of double-stranded RNA in particles of Penicillium stoloniferum virus S. Nucleic Acids Res. 1975 Oct;2(10):1889–1902. doi: 10.1093/nar/2.10.1889. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Bussey H. Effects of yeast killer factor on sensitive cells. Nat New Biol. 1972 Jan 19;235(55):73–75. doi: 10.1038/newbio235073a0. [DOI] [PubMed] [Google Scholar]
  7. Chow N. L., Shatkin A. J. Blocked and unblocked 5' termini in reovirus genome RNA. J Virol. 1975 May;15(5):1057–1064. doi: 10.1128/jvi.15.5.1057-1064.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Dahlberg J. E. Terminal sequences of bacteriophage RNAs. Nature. 1968 Nov 9;220(5167):548–552. doi: 10.1038/220548a0. [DOI] [PubMed] [Google Scholar]
  9. Herring A. J., Bevan E. A. Virus-like particles associated with the double-stranded RNA species found in killer and sensitive strains of the yeast Saccharomyces cerevisiae. J Gen Virol. 1974 Mar;22(3):387–394. doi: 10.1099/0022-1317-22-3-387. [DOI] [PubMed] [Google Scholar]
  10. Hewlett M. J., Rose J. K., Baltimore D. 5'-terminal structure of poliovirus polyribosomal RNA is pUp. Proc Natl Acad Sci U S A. 1976 Feb;73(2):327–330. doi: 10.1073/pnas.73.2.327. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hickey E. D., Weber L. A., Baglioni C. Inhibition of initiation of protein synthesis by 7-methylguanosine-5'-monophosphate. Proc Natl Acad Sci U S A. 1976 Jan;73(1):19–23. doi: 10.1073/pnas.73.1.19. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lemke P. A., Nash C. H. Fungal viruses. Bacteriol Rev. 1974 Mar;38(1):29–56. doi: 10.1128/br.38.1.29-56.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Roblin R. The 5'-terminus of bacteriophage R17 RNA: pppGp. J Mol Biol. 1968 Jan 14;31(1):51–61. doi: 10.1016/0022-2836(68)90053-3. [DOI] [PubMed] [Google Scholar]
  14. Rubin G. M. The nucleotide sequence of Saccharomyces cerevisiae 5.8 S ribosomal ribonucleic acid. J Biol Chem. 1973 Jun 10;248(11):3860–3875. [PubMed] [Google Scholar]
  15. Schonberg M., Silverstein S. C., Levin D. H., Acs G. Asynchronous synthesis of the complementary strands of the reovirus genome. Proc Natl Acad Sci U S A. 1971 Feb;68(2):505–508. doi: 10.1073/pnas.68.2.505. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Shatkin A. J., Both G. W. Reovirus mRNA: transcription and translation. Cell. 1976 Mar;7(3):305–313. doi: 10.1016/0092-8674(76)90159-8. [DOI] [PubMed] [Google Scholar]
  17. Vodkin M., Katterman F., Fink G. R. Yeast killer mutants with altered double-stranded ribonucleic acid. J Bacteriol. 1974 Feb;117(2):681–686. doi: 10.1128/jb.117.2.681-686.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Woods D. R., Bevan E. A. Studies on the nature of the killer factor produced by Saccharomyces cerevisiae. J Gen Microbiol. 1968 Apr;51(1):115–126. doi: 10.1099/00221287-51-1-115. [DOI] [PubMed] [Google Scholar]
  19. Young R. A., Perlman P. S. "Killer" character does not influence the transmission of mitochondrial genes in Saccharomyces cerevisiae. J Bacteriol. 1975 Oct;124(1):290–295. doi: 10.1128/jb.124.1.290-295.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Zimmern D. The 5' end group of tobacco mosaic virus RNA is m7G5' ppp5' Gp. Nucleic Acids Res. 1975 Jul;2(7):1189–1201. doi: 10.1093/nar/2.7.1189. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. al-Aidroos K., Somers J. M., Bussey H. Retention of cytoplasmic killer determinants in yeast cells after removal of mitochondrial DNA by ethidium bromide. Mol Gen Genet. 1973 May 28;122(4):323–330. doi: 10.1007/BF00269432. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES