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. 1977 Nov;4(11):3701–3713. doi: 10.1093/nar/4.11.3701

The presence of ovalbumin mRNA coding sequences in multiple restriction fragments of chicken DNA.

M T Doel, M Houghton, E A Cook, N H Carey
PMCID: PMC343194  PMID: 563592

Abstract

Chicken DNA has been digested with restriction enzymes and the size distribution of the DNA fragments containing ovalbumin specific sequences has been examined after separation of the fragments on agarose gels and transfer to nitrocellulose sheets. Hybridisation with terminally 32P-labelled ovalbumin mRNA fragments or with RNA populations transcribed from the DNA of a hybrid plasmid containing ovalbumin sequences was used to locate the DNA fragments coding for ovalbumin. Digestion with enzymes which do not cut within the portion of the ovalbumin gene synthesised from ovalbumin messenger RNA in vitro has shown the presence of several defined fragments carrying ovalbumin specific sequences. Possible explanations of these observations are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson J. N., Schimke R. T. Partial purification of the ovalbumin gene. Cell. 1976 Mar;7(3):331–338. doi: 10.1016/0092-8674(76)90162-8. [DOI] [PubMed] [Google Scholar]
  2. Berget S. M., Moore C., Sharp P. A. Spliced segments at the 5' terminus of adenovirus 2 late mRNA. Proc Natl Acad Sci U S A. 1977 Aug;74(8):3171–3175. doi: 10.1073/pnas.74.8.3171. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brack C., Eberle H., Bickle T. A., Yuan R. Mapping of recognition sites for the restriction endonuclease from Escherichia coli K12 on bacteriophage PM2 DNA. J Mol Biol. 1976 Dec 15;108(3):583–593. doi: 10.1016/s0022-2836(76)80138-6. [DOI] [PubMed] [Google Scholar]
  4. Celma M. L., Dhar R., Pan J., Weissman S. M. Comparison of the nucleotide sequence of the messenger RNA for the major structural protein of SV40 with the DNA sequence encoding the amino acids of the protein. Nucleic Acids Res. 1977 Aug;4(8):2549–2550. [PMC free article] [PubMed] [Google Scholar]
  5. Doel M. T., Carey N. H. The translational capacity of deadenylated ovalbumin messenger RNA. Cell. 1976 May;8(1):51–58. doi: 10.1016/0092-8674(76)90184-7. [DOI] [PubMed] [Google Scholar]
  6. Efstratiadis A., Kafatos F. C., Maxam A. M., Maniatis T. Enzymatic in vitro synthesis of globin genes. Cell. 1976 Feb;7(2):279–288. doi: 10.1016/0092-8674(76)90027-1. [DOI] [PubMed] [Google Scholar]
  7. Georgiev G. P., Ilyin Y. V., Ryskov A. P., Tchurikov N. A., Yenikolopov G. N., Gvozdev V. A., Ananiev E. V. Isolation of eukaryotic DNA fragments containing structural genes and the adjacent sequences. Science. 1977 Jan 28;195(4276):394–397. doi: 10.1126/science.401545. [DOI] [PubMed] [Google Scholar]
  8. Glover D. M., Hogness D. S. A novel arrangement of the 18S and 28S sequences in a repeating unit of Drosophila melanogaster rDNA. Cell. 1977 Feb;10(2):167–176. doi: 10.1016/0092-8674(77)90212-4. [DOI] [PubMed] [Google Scholar]
  9. Harris S. E., Means A. R., Mitchell W. M., O'Malley B. W. Synthesis of (3H)DNA complementary to ovalbumin messenger RNA: evidence for limited copies of the ovalbumin gene in chick oviduct. Proc Natl Acad Sci U S A. 1973 Dec;70(12):3776–3780. doi: 10.1073/pnas.70.12.3776. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Humphries P., Cochet M., Krust A., Gerlinger P., Kourilsky P., Chambon P. Molecular cloning of extensive sequences of the in vitro synthesized chicken ovalbumin structural gene. Nucleic Acids Res. 1977 Jul;4(7):2389–2406. doi: 10.1093/nar/4.7.2389. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. KIRBY K. S. A new method for the isolation of deoxyribonucleic acids; evidence on the nature of bonds between deoxyribonucleic acid and protein. Biochem J. 1957 Jul;66(3):495–504. doi: 10.1042/bj0660495. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kirby K. S., Cook E. A. Isolation of deoxyribonucleic acid from mammalian tissues. Biochem J. 1967 Jul;104(1):254–257. doi: 10.1042/bj1040254. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Monahan J. J., McReynolds L. A., O'Malley B. W. The ovalbumin gene. In vitro enzymatic synthesis and characterization. J Biol Chem. 1976 Dec 10;251(23):7355–7362. [PubMed] [Google Scholar]
  14. Monahan J. J., Woo S. L., Liarakos C. D., O'Malley B. W. Ovalbumin gene. Action of restriction endonucleases upon DNA coding sequence. J Biol Chem. 1977 Jul 10;252(13):4722–4728. [PubMed] [Google Scholar]
  15. Palmiter R. D., Moore P. B., Mulvihill E. R. A significant lag in the induction of ovalbumin messenger RNA by steroid hormones: a receptor translocation hypothesis. Cell. 1976 Aug;8(4):557–572. doi: 10.1016/0092-8674(76)90224-5. [DOI] [PubMed] [Google Scholar]
  16. Shapiro D. J., Schimke R. T. Immunochemical isolation and characterization of ovalbumin messenger ribonucleic acid. J Biol Chem. 1975 Mar 10;250(5):1759–1764. [PubMed] [Google Scholar]
  17. Sharp P. A., Sugden B., Sambrook J. Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis. Biochemistry. 1973 Jul 31;12(16):3055–3063. doi: 10.1021/bi00740a018. [DOI] [PubMed] [Google Scholar]
  18. Smith H. O., Wilcox K. W. A restriction enzyme from Hemophilus influenzae. I. Purification and general properties. J Mol Biol. 1970 Jul 28;51(2):379–391. doi: 10.1016/0022-2836(70)90149-x. [DOI] [PubMed] [Google Scholar]
  19. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  20. Sullivan D., Palacios R., Stavnezer J., Taylor J. M., Faras A. J., Kiely M. L., Summers N. M., Bishop J. M., Schimke R. T. Synthesis of a deoxyribonucleic acid sequence complementary to ovalbumin messenger ribonucleic acid and quantification of ovalbumin genes. J Biol Chem. 1973 Nov 10;248(21):7530–7539. [PubMed] [Google Scholar]
  21. Woo S. L., Rosen J. M., Liarakos C. D., Choi Y. C., Busch H., Means A. R., O'Malley Physical and chemical characterization of purified ovalbumin messenger RNA. J Biol Chem. 1975 Sep 10;250(17):7027–7039. [PubMed] [Google Scholar]
  22. Woo S. L., Smith R. G., Means A. R., O'Malley B. W. The ovalbumin gene. Partial purification of the coding strand. J Biol Chem. 1976 Jul 10;251(13):3868–3874. [PubMed] [Google Scholar]

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