Skip to main content
PMC home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1977 Dec;4(12):4063–4075. doi: 10.1093/nar/4.12.4063

Detection of nucleoside Q precursor in methyl-deficient E.coli tRNA.

N Okada, T Yasuda, S Nishimura
PMCID: PMC343226  PMID: 341083

Abstract

32P-Labeled tRNAAsn was isolated from methyl-deficient E. coli tRNA. Nucleotide sequence analysis showed that tRNAAsn contains three derivatives of the Q nucleoside, possibly Q precursors, in addition to guanosine in the first position of the anticodon. One of the Q precursors was isolated on a large scale. Its UV spectra were identical with those of normal Q, indicating that 7-deazaguanosine structure having a side chain at position C-7 is complete in the Q precursor. No radioactivity was incorporated into Q or Q precursors from either [methyl-14C]methionine, [1-14C]methionine or [U-14C]methionine, showing that methionine was not directly involved in the formation of Q.

Full text

PDF
4067

Images in this article

4067Image
on p.4067
4068Image
on p.4068
4069Image
on p.4069
4070Image
on p.4070
4071Image
on p.4071

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Elstner E. F., Suhadolnik R. J. The biosynthesis of the nucleoside antibiotics. IX. Purification and properties of guanosine triphosphate 8-formylhydrolase that catalyzes production of formic acid from the ureido carbon of guanosine triphosphate. J Biol Chem. 1971 Nov 25;246(22):6973–6981. [PubMed] [Google Scholar]
  2. Farkas W. R., Singh R. D. Guanylation of transfer ribonucleic acid by a cell-free lysate of rabbit reticulocytes. J Biol Chem. 1973 Nov 25;248(22):7780–7785. [PubMed] [Google Scholar]
  3. Goodman H. M., Abelson J. N., Landy A., Zadrazil S., Smith J. D. The nucleotide sequences of tyrosine transfer RNAs of Escherichia coli. Eur J Biochem. 1970 Apr;13(3):461–483. doi: 10.1111/j.1432-1033.1970.tb00950.x. [DOI] [PubMed] [Google Scholar]
  4. Harada F., Nishimura S. Possible anticodon sequences of tRNA His , tRNA Asm , and tRNA Asp from Escherichia coli B. Universal presence of nucleoside Q in the first postion of the anticondons of these transfer ribonucleic acids. Biochemistry. 1972 Jan 18;11(2):301–308. doi: 10.1021/bi00752a024. [DOI] [PubMed] [Google Scholar]
  5. Ikemura T., Dahlberg J. E. Small ribonucleic acids of Escherichia coli. I. Characterization by polyacrylamide gel electrophoresis and fingerprint analysis. J Biol Chem. 1973 Jul 25;248(14):5024–5032. [PubMed] [Google Scholar]
  6. Kasai H., Kuchino Y., Nihei K., Nishimura S. Distribution of the modified nucleoside Q and its derivatives in animal and plant transfer RNA's. Nucleic Acids Res. 1975 Oct;2(10):1931–1939. doi: 10.1093/nar/2.10.1931. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kasai H., Oashi Z., Harada F., Nishimura S., Oppenheimer N. J., Crain P. F., Liehr J. G., von Minden D. L., McCloskey J. A. Structure of the modified nucleoside Q isolated from Escherichia coli transfer ribonucleic acid. 7-(4,5-cis-Dihydroxy-1-cyclopenten-3-ylaminomethyl)-7-deazaguanosine. Biochemistry. 1975 Sep 23;14(19):4198–4208. doi: 10.1021/bi00690a008. [DOI] [PubMed] [Google Scholar]
  8. Katze J. R., Mosteller R. D. Inhibition of nucleoside Q formation in transfer ribonucleic acid during methionine starvation of relaxed-control Escherichia coli. J Bacteriol. 1976 Jan;125(1):205–210. doi: 10.1128/jb.125.1.205-210.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kitchingman G. R., Fournier M. J. In vivo maturation of an undermodified Escherichia coli leucine transfer RNA. Biochem Biophys Res Commun. 1976 Nov 22;73(2):314–322. doi: 10.1016/0006-291x(76)90709-9. [DOI] [PubMed] [Google Scholar]
  10. Kuchino Y., Kasai H., Nihei K., Nishimura S. Biosynthesis of the modified nucleoside Q in transfer RNA. Nucleic Acids Res. 1976 Feb;3(2):393–398. doi: 10.1093/nar/3.2.393. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Mirzabekov A. D., Griffin B. E. 5 s RNA conformation. Studies of its partial T 1 ribonuclease digestion by gel electrophoresis and two-dimensional thin-layer chromatography. J Mol Biol. 1972 Dec 30;72(3):633–643. doi: 10.1016/0022-2836(72)90181-7. [DOI] [PubMed] [Google Scholar]
  12. Nishimura S. Minor components in transfer RNA: their characterization, location, and function. Prog Nucleic Acid Res Mol Biol. 1972;12:49–85. [PubMed] [Google Scholar]
  13. Nishimura S., Taya Y., Kuchino Y., Oashi Z. Enzymatic synthesis of 3-(3-amino-3-carboxypropyl)uridine in Escherichia coli phenylalanine transfer RNA: transfer of the 3-amino-acid-3-carboxypropyl group from S-adenosylmethionine. Biochem Biophys Res Commun. 1974 Apr 8;57(3):702–708. doi: 10.1016/0006-291x(74)90603-2. [DOI] [PubMed] [Google Scholar]
  14. Ohashi K., Harada F., Ohashi Z., Nishimura S., Stewart T. S., Vogeli G., McCutchan T., Soll D. The nucleotide sequence of asparagine tRNA from Escherichia coli. Nucleic Acids Res. 1976 Dec;3(12):3369–3376. doi: 10.1093/nar/3.12.3369. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Okada N., Harada F., Nishimura S. Specific replacement of Q base in the anticodon of tRNA by guanine catalyzed by a cell-free extract of rabbit reticulocytes. Nucleic Acids Res. 1976 Oct;3(10):2593–2603. doi: 10.1093/nar/3.10.2593. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES