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. 1977 Dec;4(12):4165–4174. doi: 10.1093/nar/4.12.4165

End labeling of enzymatically decapped mRNA.

A Efstratiadis, J N Vournakis, H Donis-Keller, G Chaconas, D K Dougall, F C Kafatos
PMCID: PMC343232  PMID: 202926

Abstract

A method is presented for rapid and efficient 5' end labeling with 32P of capped mRNAs, by a series of three enzymatic reactions: the blocking nucleotide of the cap structure is removed by tobacco acid pyrophosphatase, and after dephosphorylation with alkaline phosphatase the 5' end is labeled with gamma-32-P-ATP and T4 polynucleotide kinase.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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