Abstract
In Bacillus subtilis SMYW cytidine and uridine are transported by a common system. Transport of these substances requires metabolic energy. After 60 sec of 3II-cytidine-uptake practically all accumulated radioactivity was found in phosphorylated products. Addition of ribonucleosides with inhibitory effect upon cytidine-uptake resulted in competitive type of inhibition while interference with deoxyribonucleosides was of hyperbolic competitive type. Adenosine possesses a high affinity to cytidine-transporting site but requires different system(s) for its own transport. Guanosine, adenine, cytosine and 5-nucleotides do not interfere with cytidine-uptake.
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- Beebe J. L. Transport alterations in a phosphatidylethanolamine-deficient mutant of Bacillus subtilis. J Bacteriol. 1972 Feb;109(2):939–942. doi: 10.1128/jb.109.2.939-942.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Butterworth P. J. The use of Dixon plots to study enzyme inhibition. Biochim Biophys Acta. 1972 Dec 7;289(2):251–253. doi: 10.1016/0005-2744(72)90074-5. [DOI] [PubMed] [Google Scholar]
- DIXON M. The determination of enzyme inhibitor constants. Biochem J. 1953 Aug;55(1):170–171. doi: 10.1042/bj0550170. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fucík V., Zadrazil S., Jurovcík M., Sormová Z. Mechanism of resistance to 5-azacytidine in Bacillus subtilis. I. Isolation and some properties of mutants resistant to 5-azacytidine and 5-aza-2'-deoxycytidine. Folia Microbiol (Praha) 1972;17(6):517–521. doi: 10.1007/BF02872738. [DOI] [PubMed] [Google Scholar]
- Gross W., Geck P., Burkhardt K. L., Ring K. Kinetic analysis of two component systems in transmembrane transport (multiple forms of transport systems). Biophysik. 1972;8(4):271–279. doi: 10.1007/BF01190612. [DOI] [PubMed] [Google Scholar]
- Komatsu Y. Adenosine uptake by isolated membrane vesicles from Escherichia coli K-12. Biochim Biophys Acta. 1973 Dec 13;330(2):206–221. doi: 10.1016/0005-2736(73)90226-5. [DOI] [PubMed] [Google Scholar]
- Komatsu Y., Tanaka K. Deoxycytidine uptake by isolated membrane vesicles from Escherichia coli K 12. Biochim Biophys Acta. 1973 Jul 18;311(4):496–506. doi: 10.1016/0005-2736(73)90125-9. [DOI] [PubMed] [Google Scholar]
- Kotyk A. Mechanisms of nonelectrolyte transport. Biochim Biophys Acta. 1973 Sep 10;300(2):183–210. doi: 10.1016/0304-4157(73)90004-x. [DOI] [PubMed] [Google Scholar]
- Peterson R. N., Boniface J., Koch A. L. Energy requirements, interactions and distinctions in the mechanisms for transport of various nucleosides in Escherichia coli. Biochim Biophys Acta. 1967 Sep 9;135(4):771–783. doi: 10.1016/0005-2736(67)90108-3. [DOI] [PubMed] [Google Scholar]
- Peterson R. N., Koch A. L. The relationship of adenosine and inosine transport in Escherichia coli. Biochim Biophys Acta. 1966 Sep 5;126(1):129–145. doi: 10.1016/0926-6585(66)90043-4. [DOI] [PubMed] [Google Scholar]
- Richardson A. G., Pierson D. L., Leach F. R. The effect of phenethyl alcohol on Bacillus subtilis transformation. II. Transport of DNA and precursors. Biochim Biophys Acta. 1969 Jan 21;174(1):276–281. doi: 10.1016/0005-2787(69)90251-2. [DOI] [PubMed] [Google Scholar]
- Roy-Burman S., Visser D. W. Transport studies of showdomycin, nucleosides and sugars in Escherichia coli B and in showdomycin-resistant mutants. Biochim Biophys Acta. 1972 Sep 1;282(1):383–392. doi: 10.1016/0005-2736(72)90343-4. [DOI] [PubMed] [Google Scholar]
- Spizizen J. TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS SUBTILIS BY DEOXYRIBONUCLEATE. Proc Natl Acad Sci U S A. 1958 Oct 15;44(10):1072–1078. doi: 10.1073/pnas.44.10.1072. [DOI] [PMC free article] [PubMed] [Google Scholar]
- von Dippe P. J., Roy-Burman S., Visser D. W. Transport of uridine in Escherichia coli B and a showdomycin-resistant mutant. Biochim Biophys Acta. 1973 Aug 9;318(1):105–112. doi: 10.1016/0005-2736(73)90340-4. [DOI] [PubMed] [Google Scholar]