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. 1974 Nov;1(11):1549–1559. doi: 10.1093/nar/1.11.1549

Inhibition of poly(A) polymerase by rifamycin derivatives

Samson T Jacob 1, Kathleen M Rose 1
PMCID: PMC343433  PMID: 10793710

Abstract

The effect of several rifamycin derivatives on poly(A) synthesis in vitro was tested using purified rat liver mitochondrial poly(A) polymerase assayed with an exogenous primer. When used at a concentration of 300 μg/ml, derivatives AF/013, PR/19, AF/AETP, M/88 and AF/ABDP completely inhibited activity corresponding to 50 μg of enzyme protein. Under similar conditions, derivatives DMAO and AF/MO failed to inhibit enzyme activity. Studies with PR/19 showed that the drug interacted directly with the enzyme molecule and did not affect the enzyme-primer complex formation. The inhibition by the drug could be reversed by increasing the substrate (ATP) concentration. It is concluded that some rifamycin derivatives can specifically inhibit template-independent nucleotide chain elongation reactions.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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