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. 1975 Jul;2(7):1163–1176. doi: 10.1093/nar/2.7.1163

Hybridization of polyuridylic acid to human DNA immobilized onto nitrocellulose filters.

R W Pero, T Bryngelsson, C Bryngelsson, A Deutsch, A Nordén, A Norgren
PMCID: PMC343503  PMID: 1153334

Abstract

The level of deoxyadenylate (da) regions in human DNA was estimated from formation of poly(U)-poly(da) triplexes on nitrocellulose filters that were RNAase resistant. The (dA) rich sequences were determined following mild ribonuclease treatment of the poly(U)-DNA hybrids (5 mug/ml at 25 degreesC for 30 min), where as exhaustive ribonuclease treatment (5 mug/ml at 25 degrees C for 6 hr) estimated the more (dA) pure sequences. The level of (dA) rich regions was 0.39% of the DNA and for the more (dA) pure regions it was 0.07%. The (dA) regions were widely distributed throughout human DNA regardless of base composition or sequence repetition. However, a concentration of (dA) regions into main band CsC1 gradient fractions of DNA and into repeated DNA was observed.

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Selected References

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